Characterization of IncX plasmids carrying quinolone and beta-lactam resistance genes in Enterobacteriaceae from various sources and geographic areas
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F15%3A43873865" target="_blank" >RIV/62157124:16270/15:43873865 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Characterization of IncX plasmids carrying quinolone and beta-lactam resistance genes in Enterobacteriaceae from various sources and geographic areas
Original language description
The aim of the study was to map the diversity of IncX plasmids carrying resistance genes to quinolones and beta-lactams in non-related Enterobacteriaceae from various sources and geographic areas and to identify the most prevalent types of these plasmids. A collection of 1800 Enterobacteriaceae isolates resistant to cefotaxime (2 mg/L) or with reduced susceptibility to ciprofloxacin (0.05 mg/L) were tested for IncX1-X4 plasmids using PCR. Isolates were tested for antibiotic resistance genes by PCR and sequencing. Isolates were identified using biochemical tests or MALDI-TOF and their relatedness was determined by pulsed-field gel electrophoresis (PFGE). Plasmid analysis included conjugation and transformation, sizing using S1-nuclease and PFGE, restriction analysis and hybridization. A total of 190 Enterobacteriaceae isolates (10.6%, n=1800) harboured at least one IncX subgroup. IncX1 subgroup was the most prevalent (107 isolates), followed by IncX2 (47), IncX4 (28) and IncX3 (17). Inc
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
Sborník příspěvků XVII. konference mladých vědeckých pracovníků s mezinárodní účastí
ISBN
978-80-7305-758-9
ISSN
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e-ISSN
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Number of pages
3
Pages from-to
75-77
Publisher name
Veterinární a farmaceutická univerzita Brno
Place of publication
Brno
Event location
Brno
Event date
May 27, 2015
Type of event by nationality
EUR - Evropská akce
UT code for WoS article
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