Comparison of Immunofluorescence Method with Histochemical and ELISA Methods Focusing on Wheat Protein Detection in Meat Products
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F17%3A43874683" target="_blank" >RIV/62157124:16270/17:43874683 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1080/09540105.2017.1328661" target="_blank" >https://doi.org/10.1080/09540105.2017.1328661</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1080/09540105.2017.1328661" target="_blank" >10.1080/09540105.2017.1328661</a>
Alternative languages
Result language
angličtina
Original language name
Comparison of Immunofluorescence Method with Histochemical and ELISA Methods Focusing on Wheat Protein Detection in Meat Products
Original language description
Gliadin is a major allergen causing allergies occurring also in meat products. Since wheat protein is used as a meat substitute to reduce cost of meat products. Sensitive consumers of these products are really threatened by food allergies in different allergic reaction. The objective of the study was to compare the histochemical, immunochemical (ALERT gliadin screening test) and immunofluorescence methods for the detection of wheat protein in model meat samples and meat products. The limit of detection (LOD) for the ALERT gliadin screening test was 10*104 mg.kg-1 of addition, while the histochemical method demonstrated concentration of wheat protein already from 10*103 mg.kg-1, and the immunofluorescence method from the concentration of 20 mg.kg-1. Comparison of the methods using McNemar?s test shows a statistically highly significant difference (p = 0.01) between the immunofluorescence method and ELISA and a statistically highly significant difference (p = 0.01) between the immunofluorescence and histochemical methods.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40500 - Other agricultural sciences
Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Food and Agricultural Immunology
ISSN
0954-0105
e-ISSN
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Volume of the periodical
28
Issue of the periodical within the volume
6
Country of publishing house
GB - UNITED KINGDOM
Number of pages
11
Pages from-to
1094-1104
UT code for WoS article
000410806400014
EID of the result in the Scopus database
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