Enterobacteriaceae isolates resistant to beta-lactam antibiotics from chicken farms in Paraguay
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F18%3A43876694" target="_blank" >RIV/62157124:16270/18:43876694 - isvavai.cz</a>
Alternative codes found
RIV/62157124:16810/18:43876694
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Enterobacteriaceae isolates resistant to beta-lactam antibiotics from chicken farms in Paraguay
Original language description
Beta-lactam antibiotics are clinically very important since they are used for treatment of various infections in humans and animals. However, resistance to these antibiotics among Enterobacteriaceae isolates is increasing as a result of dissemination of beta-lactamases that have ability to degrade betalactam antibiotics. Beta-lactamases are diverse group of enzymes with various activities but extendedspectrum beta-lactamases (ESBL) and AmpC-type beta-lactamases (AmpC) are among the most important resistance mechanisms. We aim to evaluate the occurrence of ESBL/AmpC-producing Enterobacteriaceae isolates in twelve chicken farms in Paraguay. We cultivated 66 cloacal swabs on MacConkey agar with cefoxime (2 mg/l) to select resistant isolates. Each isolate was identified using MALDI-TOF and tested for susceptibility to selected antibiotics. Afterwards we performed polymerase chain reaction (PCR) and Sanger sequencing to detect resistance genes and to assign them to a specific variant. High prevalence of ESBL/AmpC-producing Escherichia coli and Klebsiella pneumoniae isolates was found and most of them carried blaCTX-M-2, blaCTX-M-8 and blaCMY-2 genes. Transfer of these genes by plasmid conjugation experiments revealed that they are carried by plasmid which means they can be easily spread among bacterial population by horizontal gene transfer.
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
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OECD FORD branch
40301 - Veterinary science
Result continuities
Project
<a href="/en/project/LQ1601" target="_blank" >LQ1601: CEITEC 2020</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
SBORNÍK PŘÍSPĚVKŮ: XX. Konference mladých vědeckých pracovníků s mezinárodní účastí konaná v roce 100. výročí založení Vysoké školy zvěrolékařské
ISBN
978-80-7305-799-2
ISSN
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e-ISSN
neuvedeno
Number of pages
3
Pages from-to
61-63
Publisher name
Veterinární a farmaceutická univerzita Brno
Place of publication
Brno
Event location
Brno
Event date
May 30, 2018
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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