The impact of antidepressant on gene expression in Danio rerio embryos

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F18%3A43876969" target="_blank" >RIV/62157124:16270/18:43876969 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

The impact of antidepressant on gene expression in Danio rerio embryos

Original language description

Zebrafish (Danio rerio) embryos in different stages of development were used for assessment of venlafaxine antidepressant impact on non-target organisms. Two different concentrations of venlafaxine were chosen, the 300 ng/l representing the environmental concentrations (low) and the 30 ?g/l as 100x higher (high) concentration for evaluation of dose depending effect. For the gene expression, CYP1A and GST gene and two multidrug resistance proteins coded by genes ABCC1 and ABCB4 gene were selected, each of them representing one of the phase zero to III of biotransformation. The results of assay showed that the impact of venlafaxine on the zebrafish embryos is the most evident in the time of hatching (96 hours post fertilization). In this time, the results of gene expression showed increase in mRNA amount of ABCB4 and GST in both concentrations of venlafaxine. The CYP1A and ABCC1 gene revealed increase in high concentration of venlafaxine; in contrast, the low concentration for these genes revealed the decrease in mRNA amount. The second increase in gene expression was observed in 144 hours post fertilization for both concentrations of venlafaxine and all genes. The study showed that venlafaxine can affect the gene expression of biotransformation enzymes of Danio rerio embryos already in environmental concentration.

Czech name

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Czech description

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Type

D - Article in proceedings

CEP classification

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OECD FORD branch

40301 - Veterinary science

Project

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Continuities

S - Specificky vyzkum na vysokych skolach

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Water &amp; Fish 8th International Conference

ISBN

978-86-7834-308-7

ISSN

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e-ISSN

neuvedeno

Number of pages

5

Pages from-to

181-185

Publisher name

Faculty of Agriculture, University of Belgrade, Serbia

Place of publication

Belgrade

Event location

Bělehrad

Event date

Jun 13, 2018

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

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