Growth and toxin production of Bacillus cereus strains in reconstituted initial infant milk formula

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F18%3A43877052" target="_blank" >RIV/62157124:16270/18:43877052 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14310/18:00103467

Result on the web

<a href="http://dx.doi.org/10.1016/j.foodcont.2017.05.006" target="_blank" >http://dx.doi.org/10.1016/j.foodcont.2017.05.006</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.foodcont.2017.05.006" target="_blank" >10.1016/j.foodcont.2017.05.006</a>

Result language

angličtina

Original language name

Growth and toxin production of Bacillus cereus strains in reconstituted initial infant milk formula

Original language description

This study evaluates the growth and toxin production potential of B. cereus in reconstituted dried infant milk formula. Powdered milk was inoculated with approximately 1 log and 3 log cfu g-1 of spore suspension of toxigenic B. cereus strains and then stored as reconstituted milk for 48 hours at 8 °C, 15 °C, and 24 °C. The growth of B. cereus, tested by the plate method, and production of the emetic toxin and enterotoxins, tested by the gold-labelled immunosorbent assay, were regularly detected during 48 h of storage. The Baranyi-Roberts model was used to study the growth potential of B. cereus strains. In reconstituted infant milk, B. cereus did not grow at 8 °C during 48 h of storage. B. cereus multiplied and formed toxins only at 15 °C and 24 °C. The growth curve of the bacteria stored at 15 °C included the lag and exponential phases, and the growth was not completed during the period. The growth of B. cereus at 24 °C was completed, peaking within 27 and 20 hours, with the low and high inoculum sizes, respectively. Detectable amounts of toxins were formed during the bacterial growth of around 5 log cfu ml-1 (non-hemolytic enterotoxin) and 7 log cfu ml-1 (hemolysin BL and emetic toxin). Model experiments evaluated consumer risk from B. cereus toxins resulting from extended storage of reconstituted infant milk formula at improper temperatures.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

40301 - Veterinary science

Project

—

Continuities

S - Specificky vyzkum na vysokych skolach

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Food Control

ISSN

0956-7135

e-ISSN

—

Volume of the periodical

93

Issue of the periodical within the volume

November

Country of publishing house

GB - UNITED KINGDOM

Number of pages

10

Pages from-to

334-343

UT code for WoS article

000440390200041

EID of the result in the Scopus database

2-s2.0-85019430327