The evaluation of glutathione concentration in whole blood of Holstein dairy calves

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F19%3A43878130" target="_blank" >RIV/62157124:16270/19:43878130 - isvavai.cz</a>

Result on the web

<a href="https://actavet.vfu.cz/media/pdf/actavet_2019088020129.pdf" target="_blank" >https://actavet.vfu.cz/media/pdf/actavet_2019088020129.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2754/avb201988020129" target="_blank" >10.2754/avb201988020129</a>

Result language

angličtina

Original language name

The evaluation of glutathione concentration in whole blood of Holstein dairy calves

Original language description

The aim of this study was to evaluate the concentrations of total (T-GSH), oxidized (GSSG) and reduced glutathione (GSH) in blood of clinically healthy calves, to assess the effect of sex and age together with the type of feeding on its concentrations, and to assess the relationships between glutathione and metabolic indices. A total of 117 Holstein calves at the age of 1 day to 6 months were divided according to age and together with the type of feeding into 4 groups: colostrum and transition milk (n = 20), native milk (n = 39), milk replacer (n = 29) and total mixed ration (n = 29). Blood serum was used for the assessment of the metabolic profile of calves. The concentrations of individual forms of glutathione (mean +/- SEM) were as follows: T-GSH: 789.1 +/- 19.4 mu mol/l, GSSG: 54.2 +/- 4.3 mu mol/l, GSH: 681.6 +/- 15.3 mu mol/l, and GSH/GSSG: 41.1 +/- 5.9, with the coefficients of variability of 26.6%, 85.3%, 24.3% and 157.4%, respectively. Age and the type of feeding affected GSSG (P &lt;= 0.01) and GSH/GSSG (P &lt;= 0.01). The relationships between glutathione and biochemical parameters were mostly weak. T-GSH correlated with creatinine (P &lt;= 0.05) and chlorides (P &lt;= 0.05); GSSG correlated with albumin (P &lt;= 0.01), creatinine (P &lt;= 0.01), cholesterol (P &lt;= 0.05), aspartate aminotransferase (P &lt;= 0.01), alanine aminotransferase (P &lt;= 0.01), gamma-glutamyltransferase (P &lt;= 0.05) and calcium (P &lt;= 0.05); GSH/GSSG ratio correlated with creatinine (P &lt;= 0.01), cholesterol (P &lt;= 0.05), aspartate aminotransferase (P &lt;= 0.05), gamma-glutamyltransferase (P &lt;= 0.01) and calcium (P &lt;= 0.05). The evaluation of individual forms of glutathione helps in estimation of redox status in healthy animals and their monitoring can detect the stress extent.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

40301 - Veterinary science

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Acta veterinaria Brno

ISSN

0001-7213

e-ISSN

—

Volume of the periodical

88

Issue of the periodical within the volume

2

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

13

Pages from-to

129-141

UT code for WoS article

000509755300001

EID of the result in the Scopus database

2-s2.0-85071386300