Plasmids in pathogenic multiresistant Escherichia coli

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F19%3A43878236" target="_blank" >RIV/62157124:16270/19:43878236 - isvavai.cz</a>

Alternative codes found

RIV/62157124:16810/19:43878236

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Plasmids in pathogenic multiresistant Escherichia coli

Original language description

Even though Escherichia coli is a gastrointestinal tract commensal, ExPEC strains (extraintestinal pathogenic E. coli) represent a serious issue to public health. Plasmids contribute to the successful dissemination of the ExPEC strains thanks to many virulence and antibiotic resistance genes they carry. In our recent study we showed that some sublineages of E. coli ST131 obtained from diverse sources including humans, animals and waste water contained specific plasmids from incompatibility group F (IncF). These plasmids have mosaic structure, they contain often more than one IncF plasmid replicon, and encode blaCTX-M genes that allows extended-spectrum beta-lactamases production. The focus of this study is to better understand the impact of plasmids on the host fitness and on a worldwide spread of these clinically important strains. To study the bacterial fitness in the presence and absence of plasmid we need to eliminate IncF plasmids from wild-type isolates. Plasmid-free cells will be obtained using plasmid constructs. These constructed plasmids carry IncF plasmid replicons which interfere with the replicons of wild-type IncF plasmids. We will use commercial plasmid pCURE2 and a new plasmid construct pCURE-F we designed to specifically target IncF replicons which are carried by the strains from our collection. The pCURE plasmids are then eliminated thanks to their instability in a non-selective medium. After we successfully eliminate the plasmids, further analyses, such as whole genome sequencing, growth curves and competition assays, will be done aiming to understand the impact of IncF plasmids on the host cell

Czech name

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Czech description

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Type

O - Miscellaneous

CEP classification

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OECD FORD branch

10606 - Microbiology

Project

<a href="/en/project/GA18-23532S" target="_blank" >GA18-23532S: Multiresistant high-risk Escherichia coli lineages: a success story of the chromosome and plasmid interplays</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů