Fitness effect of CTX-M-15-encoding IncF plasmids on their native Escherichia coli ST131 hosts

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F21%3A43879715" target="_blank" >RIV/62157124:16270/21:43879715 - isvavai.cz</a>
Alternative codes found
RIV/62157124:16810/21:43879715
Result on the web
<a href="https://fvhe.vfu.cz/files/upload/KMVP%202021_Sborn%C3%ADk.pdf" target="_blank" >https://fvhe.vfu.cz/files/upload/KMVP%202021_Sborn%C3%ADk.pdf</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Fitness effect of CTX-M-15-encoding IncF plasmids on their native Escherichia coli ST131 hosts
Original language description
Dissemination of multi-drug resistant pathogenic bacteria is usually connected with plasmid-encoded genes. However, carriage of these plasmids commonly pose a fitness cost for its host bacteria. In this study, we aim to estimate the fitness cost of large IncF plasmids carrying blaCTX-M-15 on the natural host Escherichia coli ST131 subclone H30Rx to study possible plasmid-host coevolution. Five representatives each carrying one IncF plasmid were selected. The plasmid of each isolate was eliminated using plasmid curing method. Whole genome sequencing was performed to obtain complete chromosome and plasmid sequences and to detect chromosomal mutations in plasmid-free strains. Competition assays were conducted using flow cytometry to determine relative fitness of plasmid-free strains. The study showed that IncF plasmids produce low to non-significant fitness cost in their natural host even in a non-selective environment pointing towards host-plasmid co-evolution.
Czech name
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Czech description
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Project
<a href="/en/project/GA18-23532S" target="_blank" >GA18-23532S: Multiresistant high-risk Escherichia coli lineages: a success story of the chromosome and plasmid interplays</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
Sborník příspěvků XXIII. konference mladých vědeckých pracovníků s mezinárodní účastí
ISBN
978-80-7305-851-7
ISSN
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e-ISSN
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Number of pages
3
Pages from-to
99-101
Publisher name
Veterinární univerzita Brno
Place of publication
Brno
Event location
Brno
Event date
Jun 14, 2021
Type of event by nationality
EUR - Evropská akce
UT code for WoS article
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