Fitness effects of bla(CTX-M-15)-harbouring F2:A1:B- plasmids on their native Escherichia coli ST131 H30Rx hosts

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F22%3A43880035" target="_blank" >RIV/62157124:16270/22:43880035 - isvavai.cz</a>
Alternative codes found
RIV/62157124:16810/22:43880035 RIV/00216208:11140/22:10445478 RIV/65269705:_____/22:00077567
Result on the web
<a href="https://academic.oup.com/jac/article-abstract/77/11/2960/6650071?redirectedFrom=fulltext&login=true" target="_blank" >https://academic.oup.com/jac/article-abstract/77/11/2960/6650071?redirectedFrom=fulltext&login=true</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1093/jac/dkac250" target="_blank" >10.1093/jac/dkac250</a>

Result language
angličtina
Original language name
Fitness effects of bla(CTX-M-15)-harbouring F2:A1:B- plasmids on their native Escherichia coli ST131 H30Rx hosts
Original language description
Objectives To investigate the fitness effects of large bla(CTX-M-15)-harbouring F2:A1:B- plasmids on their native Escherichia coli ST131 H30Rx hosts. Methods We selected five E. coli ST131 H30Rx isolates of diverse origin, each carrying an F2:A1:B- plasmid with the bla(CTX-M-15) gene. The plasmid was eliminated from each isolate by displacement using an incompatible curing plasmid, pMDP5_cureEC958. WGS was performed to obtain complete chromosome and plasmid sequences of original isolates and to detect chromosomal mutations in 'cured' clones. High-throughput competition assays were conducted to determine the relative fitness of cured clones compared with the corresponding original isolates. Results We were able to successfully eliminate the F2:A1:B- plasmids from all five original isolates using pMDP5_cureEC958. The F2:A1:B- plasmids produced non-significant fitness effects in three isolates and moderate reductions in relative fitness (3%-4%) in the two remaining isolates. Conclusions We conclude that F2:A1:B- plasmids pose low fitness costs in their E. coli ST131 H30Rx hosts. This plasmid-host fitness compatibility is likely to promote the maintenance of antibiotic resistance in this clinically important E. coli lineage.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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