Polychlorinated biphenyl toxicity in the thyroid gland of wild ungulates: an in vitro model

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F20%3A43878809" target="_blank" >RIV/62157124:16270/20:43878809 - isvavai.cz</a>

Result on the web

<a href="https://actavet.vfu.cz/89/2/0151/" target="_blank" >https://actavet.vfu.cz/89/2/0151/</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2754/avb202089020151" target="_blank" >10.2754/avb202089020151</a>

Result language

angličtina

Original language name

Polychlorinated biphenyl toxicity in the thyroid gland of wild ungulates: an in vitro model

Original language description

Polychlorinated biphenyls (PCBs) are carcinogens causing endocrine disruption. While production of PCBs is now banned, wildlife exposure still occurs due to environmental contamination. We investigated thyroid toxicity in wild ungulates using three-dimensional primary thyrocyte cultures exposed to PCB 138 for 24, 48, and 72 h at concentrations ranging within 0-3000 ng/ml. Thyrocyte viability ranged within 78.71-118.34%, 98.14-104.45%, and 84.16-106.70% in fallow deer-, mouflon-, and roe deer-derived cells, respectively. Viability decreased significantly in fallow deer (P = 0.012) and roe deer (P = 0.002) thyrocytes exposed for 48 h at 30 ng/ml. While cytotoxicity ranged within 2.36-16.37%, 3.19-9.85%, and 2.76-11.21% in fallow deer, mouflon, and roe deer, respectively, only roe deer displayed significantly higher cytotoxicity at a 3 ng/ml exposure (P &lt; 0.05) and lower cytotoxicity at 30 ng/ml (P &lt; 0.01). Exposure to 30 ng/ml for 24 and 48 h induced reactive oxygen species in fallow deer. Iodide uptake at 30 ng/ml exposure increased after 24 h in fallow and roe deer, but showed a significant drop after 48 and 72 h in fallow deer, mouflon, and roe deer. Thyroxine T-4 release at 30 ng/ml exposure decreased significantly after 48 and 72 h; 24, 48 and 72 h; and 48 h in fallow deer, mouflon, and roe deer, respectively. Our findings indicate time- and species-dependent effects of PCB on performance and thyrocyte function. Use of cell culture models reduces the number of experimental specimens, increases test species welfare and replaces whole organisms with specific target cells.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

40301 - Veterinary science

Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Acta veterinaria Brno

ISSN

0001-7213

e-ISSN

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Volume of the periodical

89

Issue of the periodical within the volume

2

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

12

Pages from-to

151-162

UT code for WoS article

000548359900006

EID of the result in the Scopus database

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