Hibernation strategy: functions of bat-derived cell lines at low temperatures

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F21%3A43879608" target="_blank" >RIV/62157124:16270/21:43879608 - isvavai.cz</a>

Result on the web

<a href="https://fvhe.vfu.cz/files/upload/KMVP%202021_Sborn%C3%ADk_1.pdf" target="_blank" >https://fvhe.vfu.cz/files/upload/KMVP%202021_Sborn%C3%ADk_1.pdf</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Hibernation strategy: functions of bat-derived cell lines at low temperatures

Original language description

Heterothermy is bat adaptive strategy of controlled regulation of their body temperature to cope with seasonal climatic extremes with unavailability of insect prey. During hibernation and daily torpor, body temperature significantly decreases. Therefore, it is essential that tissues and cells are adapted on these temperature changes. We observed phagocytic intensity, proliferation and morphology of bat macrophage cell line at different temperatures simulating body temperature during hibernation (8 °C), daily torpor (17.5 °C) and euthermy (37 °C) and survival of cells obtained from different organ under experimental temperature -20 °C in medium without externally added cryoprotectant. Bat cells are adapted for significantly decreased incubation ~ body temperature. Cells maintain viability and functions at low temperatures, but the proliferation rate and intensity of phagocytosis decrease compared to euthermic conditions. Cells at 8 and -20 °C stay round shaped in suspensionvand form a few-number cell clusters. Cells of different tissues differ in their ability to survive extreme decrease and subsequent increase of temperature. The lowest viability in vitro was observed in body core organ cells (liver), contrariwise cells close to the body surface (nerves) show high viability and rapid regrowth under optimal conditions. We conclude, bat cells are adapted to overcome low temperatures using hypometabolism.

Czech name

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Czech description

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Type

D - Article in proceedings

CEP classification

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OECD FORD branch

40301 - Veterinary science

Project

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Continuities

S - Specificky vyzkum na vysokych skolach

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

XXIlI. Konference mladých vědeckých pracovníků s mezinárodní účastí

ISBN

978-80-7305-851-7

ISSN

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e-ISSN

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Number of pages

3

Pages from-to

76-78

Publisher name

Veterinární univerzita Brno

Place of publication

Brno

Event location

Brno

Event date

Jun 14, 2021

Type of event by nationality

EUR - Evropská akce

UT code for WoS article

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