Bat-derived cells use glucose as a cryoprotectant

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081766%3A_____%2F23%3A00574836" target="_blank" >RIV/68081766:_____/23:00574836 - isvavai.cz</a>

Alternative codes found

RIV/62157124:16270/23:43880951

Result on the web

<a href="https://www.sciencedirect.com/science/article/pii/S0306456523001936?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0306456523001936?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jtherbio.2023.103652" target="_blank" >10.1016/j.jtherbio.2023.103652</a>

Result language

angličtina

Original language name

Bat-derived cells use glucose as a cryoprotectant

Original language description

Evolution of heterothermy in environments with variable temperatures has allowed bats to survive food scarcity during seasonal climatic extremes by using torpor as a hibernation strategy. The controlled reduction of body temperature and metabolism through complex behavioural and physiological adaptations at organismal, organ, cellular and molecular levels includes the ability of tissues and cells to adapt to temperature alterations. Based on the prediction that cells of different tissues cultured in vitro would differ in their ability to withstand freezing and thawing of the medium, we determined the survival rate of bat-derived cells following exposure to20 degrees C for 24 h in media with no cryoprotective agents or medium supplemented by glucose in concentration range 0-3333 mM. Cell survival rates were determined in relation to availability of glucose in the medium, organ origin, cell concentration and bat species. In general, increased glucose helped cells survive at sub-zero temperatures, though concentrations up to 80-fold higher than those found in chiropterans were needed. However, cells in glucose-free phosphate buffered saline also survived, suggesting that other mechanisms may be contributing to cell survival at low temperatures. Highest in vitro viability was observed in nervus olfactorius-derived cell cultures, with high survival rates and rapid re-growth under optimal conditions after exposure to20 degrees C. Kidney cells from different bat species showed comparable overall survival rate patterns, though smaller chiropteran species appeared to utilise lower glucose levels as a cryoprotectant than larger species. Our in vitro data provide evidence that cells of heterothermic bats can survive sub-zero temperatures and that higher glucose levels in important tissues significantly improve hibernation survival at extremely low temperatures.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10613 - Zoology

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2023

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Thermal Biology

ISSN

0306-4565

e-ISSN

1879-0992

Volume of the periodical

115

Issue of the periodical within the volume

July

Country of publishing house

GB - UNITED KINGDOM

Number of pages

12

Pages from-to

103652

UT code for WoS article

001046453700001

EID of the result in the Scopus database

2-s2.0-85164447608