Performance of bat-derived macrophages at different temperatures

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081766%3A_____%2F22%3A00561727" target="_blank" >RIV/68081766:_____/22:00561727 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14310/22:00126822 RIV/62157124:16270/22:43880181 RIV/62157124:16810/22:43880181

Result on the web

<a href="https://www.frontiersin.org/articles/10.3389/fvets.2022.978756/full" target="_blank" >https://www.frontiersin.org/articles/10.3389/fvets.2022.978756/full</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/fvets.2022.978756" target="_blank" >10.3389/fvets.2022.978756</a>

Result language

angličtina

Original language name

Performance of bat-derived macrophages at different temperatures

Original language description

Heterothermy, as a temperature-dependent physiological continuum, may affect host-pathogen interactions through modulation of immune responses. Here, we evaluated proliferation and functional performance of a macrophage cell line established from the greater mouse-eared (Myotis myotis) bat at 8, 17.5, and 37 degrees C to simulate body temperatures during hibernation, daily torpor and euthermia. Macrophages were also frozen to20 degrees C and then examined for their ability to proliferate in the immediate post-thaw period. We show that bat macrophages can proliferate at lower temperatures, though their growth rate is significantly slower than at 37 degrees C. The cells differed in their shape, size and ability to attach to the plate surface at both lower temperatures, being spheroidal and free in suspension at 8 degrees C and epithelial-like, spindle-shaped and/or spheroidal at 17.5 degrees C. While phagocytosis at temperatures of 8 and 17.5 degrees C amounted to 85.8 and 83.1% of the activity observed at 37 degrees C, respectively, full phagocytic activity was restored within minutes of translocation into a higher temperature. Bat-derived macrophages were also able to withstand temperatures of 20 degrees C in a cryoprotectant-free cultivation medium and, in the immediate post-thaw period, became viable and were able to proliferate. Our in vitro data enhance understanding of macrophage biology.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10613 - Zoology

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2022

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Frontiers in Veterinary Science

ISSN

2297-1769

e-ISSN

2297-1769

Volume of the periodical

9

Issue of the periodical within the volume

SEP

Country of publishing house

CH - SWITZERLAND

Number of pages

12

Pages from-to

978756

UT code for WoS article

000858548200001

EID of the result in the Scopus database

2-s2.0-85138833530