VIM-1-producing Enterobacter asburiae with mobile colistin resistance genes from wastewaters

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F24%3A43881579" target="_blank" >RIV/62157124:16270/24:43881579 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11140/24:10485279 RIV/62157124:16810/24:43881579 RIV/65269705:_____/24:00080420

Result on the web

<a href="https://doi.org/10.1186/s12864-024-10780-7" target="_blank" >https://doi.org/10.1186/s12864-024-10780-7</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/s12864-024-10780-7" target="_blank" >10.1186/s12864-024-10780-7</a>

Result language

angličtina

Original language name

VIM-1-producing Enterobacter asburiae with mobile colistin resistance genes from wastewaters

Original language description

Background Wastewaters are considered as important players in the spread of antimicrobial resistance, thus affecting the health of humans and animals. Here, we focused on wastewaters as a possible source of carbapenemase-producing Enterobacterales for the environment. Methods A total of 180 presumptive coliforms from hospital and municipal wastewaters, and a river in the Czech Republic were obtained by selective cultivation on meropenem-supplemented media and tested for presence of carbapenemase-encoding genes by PCR. Strains carrying genes of interest were characterized by testing antimicrobial susceptibility, carbapenemase production and combination of short- and long- read whole-genome sequencing. The phylogenetic tree including publicly available genomes of Enterobacter asburiae was conducted using Prokka, Roary and RAxML. Results Three VIM-producing Enterobacter asburiae isolates, members of the Enterobacter cloacae complex, were detected from hospital and municipal wastewaters, and the river. The blaVIM-1 gene was located within a class 1 integron that was carried by different F-type plasmids and one non-typeable plasmid. Furthermore, one of the isolates carried plasmid-borne colistin-resistance gene mcr-10, while in another isolate chromosomally located mcr-9 without colistin resistance phenotype was detected. In addition, the analysis of 685 publicly available E. asburiae genomes showed they frequently carry carbapenemase genes, highlighting the importance of this species in the emergence of resistance to last-line antibiotics. Conclusion Our findings pointed out the important contribution of hospital and community wastewaters in transmission of multi-drug resistant pathogens.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10606 - Microbiology

Project

<a href="/en/project/NU20J-09-00040" target="_blank" >NU20J-09-00040: Whole genome sequencing and metagenomics towards understanding transmission routes of antibiotic resistant bacteria from hospitals to the environment</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

BMC genomics

ISSN

1471-2164

e-ISSN

1471-2164

Volume of the periodical

25

Issue of the periodical within the volume

1

Country of publishing house

GB - UNITED KINGDOM

Number of pages

8

Pages from-to

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UT code for WoS article

001317001200001

EID of the result in the Scopus database

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