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Effect of sphingosine-1-phosphate on L-type calcium current and Ca2+ transient in rat ventricular myocytes

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16370%2F16%3A43874428" target="_blank" >RIV/62157124:16370/16:43874428 - isvavai.cz</a>

  • Result on the web

    <a href="http://dx.doi.org/10.1007/s11010-016-2752-8" target="_blank" >http://dx.doi.org/10.1007/s11010-016-2752-8</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1007/s11010-016-2752-8" target="_blank" >10.1007/s11010-016-2752-8</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Effect of sphingosine-1-phosphate on L-type calcium current and Ca2+ transient in rat ventricular myocytes

  • Original language description

    Modulation of Ca2+ homoeostasis in cardiac myocytes plays a major role in beat-to-beat regulation of heart function. Previous studies suggest that sphingosine-1-phosphate (S1P), a biologically active sphingomyelin metabolite, regulates Ca2+ handling in cardiac myocytes, but the underlying mechanism is unclear. In the present study, we tested the hypothesis that S1P-induced functional alteration of intracellular Ca2+ handling includes the L-type calcium channel current (I-Ca,I-L) via a signalling pathway involving P21-activated kinase 1 (Pak1). Our results show that, in rat ventricular myocytes, S1P (100 nM) does not affect the basal activity of I-Ca,I-L but is able to partially reverse the effect of the beta-adrenergic agonist Isoproterenol (ISO, 100 nM) on I-Ca,I-L. S1P (25 nM) also significantly prevents ISO (5 nM)-induced Ca2+ waves and diastolic Ca2+ release in these cells. Our further molecular characterisation demonstrates that Pak1 activity is increased in myocytes treated with S1P (25 nM) compared with those myocytes without treatment of S1P. By immunoprecipitation we demonstrate that Pak1 and protein phosphatase 2A (PP2A) are associated in ventricular tissue indicating their functional interaction. Thus the results indicate that S1P attenuates beta-adrenergic stress-induced alteration of intracellular Ca2+ release and L-type Ca2+ channel current at least in part via Pak1-PP2A-mediated signalling.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    FR - Pharmacology and apothecary chemistry

  • OECD FORD branch

Result continuities

  • Project

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2016

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Molecular and cellular biochemistry

  • ISSN

    0300-8177

  • e-ISSN

  • Volume of the periodical

    419

  • Issue of the periodical within the volume

    1-2

  • Country of publishing house

    NL - THE KINGDOM OF THE NETHERLANDS

  • Number of pages

    10

  • Pages from-to

    83-92

  • UT code for WoS article

    000381208500009

  • EID of the result in the Scopus database