Effect of sphingosine-1-phosphate on L-type calcium current and Ca2+ transient in rat ventricular myocytes
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16370%2F16%3A43874428" target="_blank" >RIV/62157124:16370/16:43874428 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1007/s11010-016-2752-8" target="_blank" >http://dx.doi.org/10.1007/s11010-016-2752-8</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s11010-016-2752-8" target="_blank" >10.1007/s11010-016-2752-8</a>
Alternative languages
Result language
angličtina
Original language name
Effect of sphingosine-1-phosphate on L-type calcium current and Ca2+ transient in rat ventricular myocytes
Original language description
Modulation of Ca2+ homoeostasis in cardiac myocytes plays a major role in beat-to-beat regulation of heart function. Previous studies suggest that sphingosine-1-phosphate (S1P), a biologically active sphingomyelin metabolite, regulates Ca2+ handling in cardiac myocytes, but the underlying mechanism is unclear. In the present study, we tested the hypothesis that S1P-induced functional alteration of intracellular Ca2+ handling includes the L-type calcium channel current (I-Ca,I-L) via a signalling pathway involving P21-activated kinase 1 (Pak1). Our results show that, in rat ventricular myocytes, S1P (100 nM) does not affect the basal activity of I-Ca,I-L but is able to partially reverse the effect of the beta-adrenergic agonist Isoproterenol (ISO, 100 nM) on I-Ca,I-L. S1P (25 nM) also significantly prevents ISO (5 nM)-induced Ca2+ waves and diastolic Ca2+ release in these cells. Our further molecular characterisation demonstrates that Pak1 activity is increased in myocytes treated with S1P (25 nM) compared with those myocytes without treatment of S1P. By immunoprecipitation we demonstrate that Pak1 and protein phosphatase 2A (PP2A) are associated in ventricular tissue indicating their functional interaction. Thus the results indicate that S1P attenuates beta-adrenergic stress-induced alteration of intracellular Ca2+ release and L-type Ca2+ channel current at least in part via Pak1-PP2A-mediated signalling.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FR - Pharmacology and apothecary chemistry
OECD FORD branch
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Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Molecular and cellular biochemistry
ISSN
0300-8177
e-ISSN
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Volume of the periodical
419
Issue of the periodical within the volume
1-2
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
10
Pages from-to
83-92
UT code for WoS article
000381208500009
EID of the result in the Scopus database
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