Effects of polyphenol compounds melanin on NAFLD/NASH prevention

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16370%2F17%3A43876014" target="_blank" >RIV/62157124:16370/17:43876014 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14110/17:00098633

Result on the web

<a href="http://dx.doi.org/10.1016/j.biopha.2017.01.028" target="_blank" >http://dx.doi.org/10.1016/j.biopha.2017.01.028</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.biopha.2017.01.028" target="_blank" >10.1016/j.biopha.2017.01.028</a>

Result language

angličtina

Original language name

Effects of polyphenol compounds melanin on NAFLD/NASH prevention

Original language description

Background: One of the pathogenic mechanisms of the progression non-alcoholic liver disease ( NAFLD) to nonalcoholic steatohepatitis (NASH) is the accumulation of reactive oxygen species (ROS). So, antioxidant therapy is necessary for successful treatment of the liver injury. We have paid attention to melanin produced by yeast Nadsoniella nigra strain X-1 as novel antioxidant and anti-inflammatory agents with low toxicity. In current study we aimed to investigate the preventive effect of melanin on the monosodium glutamate (MSG) induced NAFLD model in rats. Methods: The study was carried out on 45 Wistar rats that were divided into 3 groups: intact, MSG-and MSG + melanin groups (n = 15 in each group). Newborn rats of MSG-and MSG + melanin groups were administered with MSG (4 mg/g, 8 mu l/g, subcutaneously) at 2nd-10th days of life. Since the age of 1 month, rats of MSG-group were treated with water (0.25 ml/100 g), rats of MSG + melanin groups-with melanin (1 mg/kg) dissolved in water (0.25 ml/100 g). Introduction: had been performed intermittently (two-week courses alternated with two-week breaks) for 3 months. In 4-month rats anthropometrical parameters and visceral adipose tissue (VAT) mass were estimated. To assess morphological changes in liver we used NAS (NAFLD activity score). The content of pro-inflammatory cytokines (interleukin (IL)-1 beta, IL-12Bp40, interferon (INF)-gamma) and anti-inflammatory cytokines (IL-4, IL-10, tumor growth factor (TGF)-beta) were measured by ELISA. Results: We found significantly lower total score (1.0 +/- 0.19 vs 3.33 +/- 0.36, p &lt; 0.001), degree of steatosis (0.73 +/- 0.18 vs 1.80 +/- 0.17, p &lt; 0.001) and manifestation of lobular inflammation (0.27 +/- 0.11 vs 1.20 +/- 0.17, p &lt; 0.001) due to NAFLD activity score in MSG + melanin group compared to MSG-obesity. NASH we confirmed only in 33.3% of rats with MSG-obesity that was significantly higher than after melanin (6.7%) administration (p = 0.033). Melanin administration reduce amount of visceral fat on 44.5% (p 0.001) as compared to MSG-obesity group. Melanin reduced the content of IL-1 beta in rat serum and restored the level of anti-inflammatory cytokines (IL-10, TGF-beta) to the control values. Conclusion: Thus, the administration of melanin can prevent development of NAFLD/NASH in rats with MSG-induced obesity and can be considered as possible novel therapeutic agents but further studies to confirm its action needed.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

30104 - Pharmacology and pharmacy

Project

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Continuities

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biomedicine &amp; pharmacotherapy

ISSN

0753-3322

e-ISSN

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Volume of the periodical

88

Issue of the periodical within the volume

APR 2017

Country of publishing house

FR - FRANCE

Number of pages

10

Pages from-to

267-276

UT code for WoS article

000395528000031

EID of the result in the Scopus database

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