Plasmid-mediated resistance to colistin in healthy chicken in Paraguay
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16810%2F19%3A43878235" target="_blank" >RIV/62157124:16810/19:43878235 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Plasmid-mediated resistance to colistin in healthy chicken in Paraguay
Original language description
According to WHO, the antibiotic resistance is one of the major problems to deal with in our millennium. High occurrence of multi-drug resistant Gram-negative bacteria led to reintroducing less user-friendly antibiotics such as colistin to human medicine. Until the discovery of mcr-1 gene in 2016, only chromosomally-encoded resistance to colistin was observed. This type of resistance can be transferred only to other generations of bacteria. However, mcr (mobile colistin resistance) genes are plasmid-mediated and can be transferred very easily even within different Enterobacterales and cause problems in treatment of bacterial infections. We observed high prevalence (24%; 16/66) of mcr-5 gene in our samples from finisher broiler chicken from 12 different farms in Paraguay. We obtained 28 mcr-5-carrying Escherichia coli isolates and characterize them using diverse microbiological and molecular methods including whole-genome sequencing and conjugation experiments. We were the first to detect mcr-5 gene within conjugative plasmids of various types. This indicates it can be transfered more easily than it was originally thought as it was detected only within small non-conjugative plasmids before. Moreover, we observed frequent association of mcr-5 and genes encoding resistance to extendedspectrum beta-lactams in the same plasmid ? IncI1/ST113-mcr-5-blaCTX-M-8 and IncI1/ST12-mcr-5-blaCMY-2. IncI1/ST113 and IncI1/ST12 are epidemic plasmid lineages disseminating the beta-lactamase genes worldwide. Their association with mcr-5 represents a big threat for public health.
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů