Two-photon excitation with pico-second fluorescence lifetime imaging to detect nuclear association of flavanols

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62690094%3A18470%2F12%3A43867001" target="_blank" >RIV/62690094:18470/12:43867001 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.aca.2011.12.068" target="_blank" >http://dx.doi.org/10.1016/j.aca.2011.12.068</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.aca.2011.12.068" target="_blank" >10.1016/j.aca.2011.12.068</a>

Result language
angličtina
Original language name
Two-photon excitation with pico-second fluorescence lifetime imaging to detect nuclear association of flavanols
Original language description
Two-photon excitation enabled for the first time the observation and measurement of excited state fluorescence lifetimes from three flavanols ( ~1.0 ns for catechin and epicatechin, but {45 ps for epigallocatechin gallate (EGCG)). The shorter lifetime for EGCG is in line with a lower fluorescence quantum yield of 0.003 compared to catechin (0.015) and epicatechin (0.018). In vivo experiments with onion cells demonstrated that tryptophan and quercetin, which tend to be major contributors of background fluorescence in plant cells, have sufficiently low cross sections for two-photon excitation at 630 nm and therefore do not interfere with detection of externally added or endogenous flavanols in Allium cepa or Taxus baccata cells. Applying two-photon excitation to flavanols enabled 3-D fluorescence lifetime imaging microscopy and showed that added EGCG penetrated the whole nucleus of onion cells. Interestingly, EGCG and catechin showed different lifetime behaviour when bound to the nucleus
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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