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Investigation of patulin and citrinin in grape must and wine from grapes naturally contaminated by strains of Penicillium expansum

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62690094%3A18470%2F18%3A50014501" target="_blank" >RIV/62690094:18470/18:50014501 - isvavai.cz</a>

  • Alternative codes found

    RIV/75010330:_____/18:00012327

  • Result on the web

    <a href="https://www.sciencedirect.com/science/article/pii/S0278691518303958" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0278691518303958</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1016/j.fct.2018.06.022" target="_blank" >10.1016/j.fct.2018.06.022</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Investigation of patulin and citrinin in grape must and wine from grapes naturally contaminated by strains of Penicillium expansum

  • Original language description

    Twenty three strains of Penicillium expansum, as a predominant species, were isolated from 23 (92%) out of 25 grape samples of 17 different grape varieties. The results of the identification of P. expansum strains were confirmed by a PCR method. Most of the isolates of P. expansum (21/23, 91%), when tested for toxigenicity, were bi-toxigenic: they produced citrinin (CIT) and particularly high amounts of patulin (PAT). A validated UPLC-MS/ MS method for the determination of PAT and CIT was applied. The limits of quantification (LOQ) for PAT and CIT in grape must and toxigenicity testing samples were 100 and 2 ng/g, respectively. The results of PAT and CIT quantification in 23 grape must samples demonstrated the occurrence of PAT in 10 (43%) grape must samples (mean: 171 ng/g; median: 50 ng/g; and range: 143–644 ng/g) and the occurrence of CIT in two (9%) grape must samples (mean: 1 ng/g; median: 1 ng/g; and range: 2.5–3.5 ng/g). This is the first report on the natural occurrence of CIT in grape must. A validated HPLC-UV-VIS method for the determination of PAT in wine samples was applied, and concentrations in all 23 wine samples were below the LOQ (&lt; 10 ng/g).

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    30304 - Public and environmental health

Result continuities

  • Project

  • Continuities

    S - Specificky vyzkum na vysokych skolach

Others

  • Publication year

    2018

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Food and chemical toxicology

  • ISSN

    0278-6915

  • e-ISSN

  • Volume of the periodical

    118

  • Issue of the periodical within the volume

    August

  • Country of publishing house

    GB - UNITED KINGDOM

  • Number of pages

    7

  • Pages from-to

    805-811

  • UT code for WoS article

    000442714300082

  • EID of the result in the Scopus database

    2-s2.0-85048895725