Oxidative Stress and Analysis of Selected SNPs ofACHE(rs 2571598),BCHE(rs 3495),CAT(rs 7943316),SIRT1(rs 10823108),GSTP1(rs 1695), and GeneGSTM1,GSTT1in Chronic Organophosphates Exposed Groups from Cameroon and Pakistan
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62690094%3A18470%2F20%3A50017108" target="_blank" >RIV/62690094:18470/20:50017108 - isvavai.cz</a>
Result on the web
<a href="https://www.mdpi.com/1422-0067/21/17/6432" target="_blank" >https://www.mdpi.com/1422-0067/21/17/6432</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/ijms21176432" target="_blank" >10.3390/ijms21176432</a>
Alternative languages
Result language
angličtina
Original language name
Oxidative Stress and Analysis of Selected SNPs ofACHE(rs 2571598),BCHE(rs 3495),CAT(rs 7943316),SIRT1(rs 10823108),GSTP1(rs 1695), and GeneGSTM1,GSTT1in Chronic Organophosphates Exposed Groups from Cameroon and Pakistan
Original language description
The detrimental effects of organophosphates (OPs) on human health are thought to be of systemic, i.e., irreversible inhibition of acetylcholinesterase (AChE) at nerve synapses. However, several studies have shown that AChE inhibition alone cannot explain all the toxicological manifestations in prolonged exposure to OPs. The present study aimed to assess the status of antioxidants malondialdehyde (MDA), superoxide dismutase (SOD), glutathione (GSH) (reduced), catalase, and ferric reducing antioxidant power (FRAP) in chronic OP-exposed groups from Cameroon and Pakistan. Molecular analysis of genetic polymorphisms (SNPs) of glutathione transferases (GSTM1, GSTP1, GSTT1), catalase gene (CAT, rs7943316), sirtuin 1 gene (SIRT1, rs10823108), acetylcholinesterase gene (ACHE, rs2571598), and butyrylcholinesterase gene (BCHE, rs3495) were screened in the OP-exposed individuals to find the possible causative association with oxidative stress and toxicity. Cholinesterase and antioxidant activities were measured by colorimetric methods using a spectrophotometer. Salting-out method was employed for DNA extraction from blood followed by restriction fragment length polymorphism (RFLP) for molecular analysis. Cholinergic enzymes were significantly decreased in OP-exposed groups. Catalase and SOD were decreased and MDA and FRAP were increased in OP-exposed groups compared to unexposed groups in both groups. GSH was decreased only in Pakistani OPs-exposed group. Molecular analysis of ACHE, BCHE, Catalase, GSTP1, and GSTM1 SNPs revealed a tentative association with their phenotypic expression that is level of antioxidant and cholinergic enzymes. The study concludes that chronic OPs exposure induces oxidative stress which is associated with the related SNP polymorphism. The toxicogenetics of understudied SNPs were examined for the first time to our understanding. The findings may lead to a newer area of investigation on OPs induced health issues and toxicogenetics.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
ISSN
1422-0067
e-ISSN
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Volume of the periodical
21
Issue of the periodical within the volume
17
Country of publishing house
CH - SWITZERLAND
Number of pages
17
Pages from-to
"Article Number: 6432"
UT code for WoS article
000569794600001
EID of the result in the Scopus database
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