A fieldable, high-throughput, cost-efficient high performance liquid chromatography-ultraviolet absorption detection (HPLC-UV) method for the quantitation of bispyridinium quaternary aldoxime cholinesterase reactivators in blood
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62690094%3A18470%2F21%3A50018005" target="_blank" >RIV/62690094:18470/21:50018005 - isvavai.cz</a>
Result on the web
<a href="https://akjournals.com/view/journals/1326/aop/article-10.1556-1326.2020.00781/article-10.1556-1326.2020.00781.xml" target="_blank" >https://akjournals.com/view/journals/1326/aop/article-10.1556-1326.2020.00781/article-10.1556-1326.2020.00781.xml</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1556/1326.2020.00781" target="_blank" >10.1556/1326.2020.00781</a>
Alternative languages
Result language
angličtina
Original language name
A fieldable, high-throughput, cost-efficient high performance liquid chromatography-ultraviolet absorption detection (HPLC-UV) method for the quantitation of bispyridinium quaternary aldoxime cholinesterase reactivators in blood
Original language description
Mono- and bis-pyridinium quaternary aldoximes (K-oximes) have long been employed as cholinesterase reactivator components of antidotes against lethal cholinesterase-inhibiting organophosphorous chemicals. Their positive charge poses difficulties in their chromatographic analysis, resulting in the publication of different approaches for each K-oxime. A multiplexed method is presented for the rapid quantitation of 10 K-oximes in blood with its utility demonstrated in vivo. Liquid chromatography with absorbance detection was employed. Reversed-phase separation was achieved on a highly nonpolar stationary phase. Method validation was based on the respective guideline of the European Medicines Agency. Times to peak concentrations and 120-min areas under the time-concentration curves were determined in rats following intraperitoneal administration. Adequate retention and separation of K-oximes with acceptable peak shapes in short isocratic runs was achieved by adjusting ionic strength, organic content and the concentration of the ion-pairing agent of the mobile phase. Chromatographic properties were governed by optimizing the concentration of dissolved ions. Accurate adjustment of the organic content was indispensable for avoiding peak drifting and splitting. Dose-adjusted exposure to K-347 and K-868 was exceptionally low, while exposure to K-48 was the highest. The method is suitable for screening systemic exposure to various K-oximes and can be extended.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
<a href="/en/project/8F17004" target="_blank" >8F17004: Novel butyrylcholinesterase reactivators for pseudo-catalytic scavenging of organophoshates</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
ACTA CHROMATOGRAPHICA
ISSN
1233-2356
e-ISSN
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Volume of the periodical
33
Issue of the periodical within the volume
2
Country of publishing house
HU - HUNGARY
Number of pages
11
Pages from-to
134-144
UT code for WoS article
000631178700006
EID of the result in the Scopus database
2-s2.0-85104963571