Lipidomic analysis using hydrophilic interaction liquid chromatography microgradient fractionation of total lipid extracts

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62690094%3A18470%2F21%3A50018337" target="_blank" >RIV/62690094:18470/21:50018337 - isvavai.cz</a>

Alternative codes found

RIV/60162694:G44__/21:00557168

Result on the web

<a href="https://www.sciencedirect.com/science/article/pii/S0021967321005045" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0021967321005045</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.chroma.2021.462380" target="_blank" >10.1016/j.chroma.2021.462380</a>

Result language

angličtina

Original language name

Lipidomic analysis using hydrophilic interaction liquid chromatography microgradient fractionation of total lipid extracts

Original language description

Lipidomic samples are complex mixtures of structurally different species of a wide range of concen- trations providing challenges in their characterization. In this work, we present a proof of concept for the application of a simple microgradient liquid chromatography device on the detailed analysis of lipid classes. Our lipidomic analysis is based on a lipid class microgradient fractionation of a total lipid extract using an in-house-prepared hydrophilic interaction liquid chromatography microcolumn followed by RP- LC/MS of the collected lipid class fractions. The final fractionation method uses a 40-mm-long microcol- umn of 500 μm ID with silica stationary phase obtained from a commercially available chromatographic column and the microgradient of the mobile phase prepared in a microsyringe using methyl tert -butyl ether (MTBE) –methanol –water –ammonium acetate mixtures of various elution strengths. MTBE total lipid extract is directly separated by microgradient elution into lipid classes according to their polarity, which enables the collection of isolated fractions of most lipid classes. The method has been applied to the fractionation of porcine brain extract into nonpolar lipids, hexosylceramides, phosphoethanolamines, phosphocholines, sphingomyelins, and lysophosphocholines classes. Achieved repeatability, recovery, and advanced lipid coverage prove the applicability of the microgradient fractionation of total lipid extract for the comprehensive lipidomic analysis.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10406 - Analytical chemistry

Project

<a href="/en/project/GA20-12289S" target="_blank" >GA20-12289S: The coupling of supercritical fluid chromatography with mass spectrometry as a new tool for characterization of lipids and polar metabolites</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

JOURNAL OF CHROMATOGRAPHY A

ISSN

0021-9673

e-ISSN

—

Volume of the periodical

1653

Issue of the periodical within the volume

September

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

7

Pages from-to

"Article Number: 462380"

UT code for WoS article

000685249900001

EID of the result in the Scopus database

2-s2.0-85111538995