Alpha-class glutathione S-transferases involved in the detoxification of aflatoxin B1 in ducklings
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62690094%3A18470%2F23%3A50020271" target="_blank" >RIV/62690094:18470/23:50020271 - isvavai.cz</a>
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S0278691523000844?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0278691523000844?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.fct.2023.113682" target="_blank" >10.1016/j.fct.2023.113682</a>
Alternative languages
Result language
angličtina
Original language name
Alpha-class glutathione S-transferases involved in the detoxification of aflatoxin B1 in ducklings
Original language description
The objective of this study was to identify the key glutathione S-transferase (GST) isozymes involved in the detoxification of Aflatoxin B1 (AFB1) in ducks' primary hepatocytes. The full-length cDNA encoding the 10 GST isozymes (GST, GST3, GSTM3, MGST1, MGST2, MGST3, GSTK1, GSTT1, GSTO1 and GSTZ1) were isolated/synthesized from ducks' liver and cloned into the pcDNA3.1(+) vector. The results showed that pcDNA3.1(+)-GSTs plasmids were successfully transferred into the ducks' primary hepatocytes and the mRNA of the 10 GST isozymes were overexpressed by 1.9–3274.7 times. Compared to the control, 75 μg/L (IC30) or 150 μg/L (IC50) AFB1 treatment reduced the cell viability by 30.0–50.0% and increased the LDH activity by 19.8–58.2% in the ducks' primary hepatocytes. Notably, the AFB1–induced changes in cell viability and LDH activity were mitigated by overexpression of GST and GST3. Compared to the cells treated with AFB1, exo-AFB1-8,9-epoxide (AFBO)–GSH, as the major detoxified product of AFB1, was increased in the cells overexpression of GST and GST3. Moreover, the sequences, phylogenetic and domain analysis revealed that the GST and GST3 were orthologous to Meleagris gallopavo GSTA3 and GSTA4. In conclusion, this study found that the ducks' GST and GST3 were orthologous to Meleagris gallopavo GSTA3 and GSTA4, which were involved in the detoxification of AFB1 in ducks’ primary hepatocytes. © 2023 Elsevier Ltd
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30108 - Toxicology
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Food and chemical toxicology
ISSN
0278-6915
e-ISSN
1873-6351
Volume of the periodical
174
Issue of the periodical within the volume
April
Country of publishing house
GB - UNITED KINGDOM
Number of pages
9
Pages from-to
"Article number: 113682"
UT code for WoS article
000949780300001
EID of the result in the Scopus database
2-s2.0-85150058386