The effect of epigallocatechin gallate on hepatocytes isolated from normal and partially hepatectomized rats
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11150%2F14%3A10218611" target="_blank" >RIV/00216208:11150/14:10218611 - isvavai.cz</a>
Result on the web
<a href="http://www.nrcresearchpress.com/doi/abs/10.1139/cjpp-2014-0069#.U-takxAQcvk" target="_blank" >http://www.nrcresearchpress.com/doi/abs/10.1139/cjpp-2014-0069#.U-takxAQcvk</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1139/cjpp-2014-0069" target="_blank" >10.1139/cjpp-2014-0069</a>
Alternative languages
Result language
angličtina
Original language name
The effect of epigallocatechin gallate on hepatocytes isolated from normal and partially hepatectomized rats
Original language description
Epigallocatechin gallate (EGCG) is an antioxidant found in green tea. In this study, male Wistar rats were subjected either to partial hepatectomy (PHx), or a sham operation (LAP). Twenty-four hours after surgery, hepatocytes were isolated and treated with various concentrations of EGCG for up to 72 h. We then measured markers of cell viability, oxidative stress, DNA synthesis, and caspase activity. Morphological criteria, cell viability tests, and albumin synthesis revealed toxicity starting at 10 ?mol/L. DNA synthesis was higher in hepatocytes isolated from rats after PHx and inhibited by EGCG. Furthermore, EGCG increased the activity of caspases 3 and 7, seen more in hepatocytes from PHx rats. In conclusion, EGCG at a concentration of 10 ?mol/L wastoxic for hepatocytes isolated from both PHx and LAP rats.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
ED - Physiology
OECD FORD branch
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Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Canadian Journal of Physiology and Pharmacology
ISSN
0008-4212
e-ISSN
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Volume of the periodical
92
Issue of the periodical within the volume
6
Country of publishing house
CA - CANADA
Number of pages
6
Pages from-to
512-517
UT code for WoS article
000338712700013
EID of the result in the Scopus database
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