Cloning of CAT genes in Satsuma mandarin and their expression characteristics in response to environmental stress and arbuscular mycorrhizal fungi

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62690094%3A18470%2F24%3A50021445" target="_blank" >RIV/62690094:18470/24:50021445 - isvavai.cz</a>

Result on the web

<a href="https://link.springer.com/article/10.1007/s00299-024-03218-7" target="_blank" >https://link.springer.com/article/10.1007/s00299-024-03218-7</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00299-024-03218-7" target="_blank" >10.1007/s00299-024-03218-7</a>

Result language

angličtina

Original language name

Cloning of CAT genes in Satsuma mandarin and their expression characteristics in response to environmental stress and arbuscular mycorrhizal fungi

Original language description

Catalase (CAT), a tetrameric heme-containing enzyme, removes hydrogen peroxide (H2O2) to maintain low oxidativedamage in plants exposed to environmental stress. This study aimed to clone CAT genes from Citrus sinensis cv. “Oita4” and analyze their expression patterns in response to environmental stress, exogenous abscisic acid (ABA), and arbuscularmycorrhizal fungal inoculation. Two CAT genes, CitCAT1 (NCBI accession: PP067858) and CitCAT2 (NCBI accession:PP061394) were cloned, and the open reading frames of their proteins were 1479 bp and 1539 bp, respectively, each encoding492 and 512 amino acids predicted to be localized in the peroxisome, with CitCAT1 being a stable hydrophilic protein andCitCAT2 being an unstable hydrophilic protein. The similarity of their amino acid sequences reached 83.24%, and the twogenes were distantly related. Both genes were expressed in stems, leaves, flowers, and fruits, accompanied by the highestexpression in mature leaves. In addition, CitCAT1 expression was mainly up-regulated by high temperatures (37 °C), exogenousABA, and PEG stress within a short period of time, whereas CitCAT2 expression was up-regulated by exogenous ABAand low-temperature (4 °C) stress. Low temperatures (0 °C) for 12 h just up-regulated CitCAT2 expression in Diversisporaversiformis-inoculated plants, and D. versiformis inoculation up-regulated CitCAT2 expression, along with lower hydrogenperoxide and malondialdehyde levels in mycorrhizal plants at low temperatures. It is concluded that CitCAT2 has an importantrole in resistance to low temperatures as well as mycorrhizal enhancement of host resistance to low temperatures.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10611 - Plant sciences, botany

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Plant Cell Reports

ISSN

0721-7714

e-ISSN

1432-203X

Volume of the periodical

43

Issue of the periodical within the volume

5

Country of publishing house

US - UNITED STATES

Number of pages

12

Pages from-to

"Article number: 123"

UT code for WoS article

001205943600002

EID of the result in the Scopus database

2-s2.0-85190765118