Influence of LOX/COX inhibitors on cell differentation induced by all-trans retinoic acid in neuroblastoma cell lines.

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F65269705%3A_____%2F10%3A%230001023" target="_blank" >RIV/65269705:_____/10:#0001023 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Influence of LOX/COX inhibitors on cell differentation induced by all-trans retinoic acid in neuroblastoma cell lines.

Original language description

We investigated the possible modulation by LOX/ COX inhibitors of all-trans retinoic acid (ATRA)-induced cell differentiation in two established neuroblastoma cell lines, SH-SY5Y and SK-N-BE(2). Caffeic acid, as an inhibitor of 5-lipoxygenase, and celecoxib, as an inhibitor of cyclooxygenase-2, were chosen for this study. The effects of the combined treatment with ATRA and LOX/COX inhibitors on neuroblastoma cells were studied using cell morphology assessment, detection of differentiation markers by immunoblotting, measurement of proliferation activity, and cell cycle analysis and apoptosis detection by flow cytometry. The results clearly demonstrated the potential of caffeic acid to enhance ATRA-induced cell differentiation, especially in the SK-N-BE(2) cell line, whereas application of celecoxib alone or with ATRA led predominantly to cytotoxic effects in both cell lines.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FD - Oncology and haematology

OECD FORD branch

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Project

<a href="/en/project/NR9341" target="_blank" >NR9341: Modulation of antineoplastic effect of retinoids by LOX/COX inhibitors on the model of solid childhood tumors: in vitro study</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2010

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

International Journal of Molecular Medicine

ISSN

1107-3756

e-ISSN

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Volume of the periodical

25

Issue of the periodical within the volume

2

Country of publishing house

GR - GREECE

Number of pages

9

Pages from-to

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UT code for WoS article

000273862300013

EID of the result in the Scopus database

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