In vitro activation of CMV-specific human CD8(+) T cells by adenylate cyclase toxoids delivering pp65 epitopes

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F65269705%3A_____%2F12%3A%230001441" target="_blank" >RIV/65269705:_____/12:#0001441 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14110/12:00059406 RIV/65269705:_____/12:#0001816

Result on the web

<a href="http://dx.doi.org/10.1038/bmt.2011.68" target="_blank" >http://dx.doi.org/10.1038/bmt.2011.68</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/bmt.2011.68" target="_blank" >10.1038/bmt.2011.68</a>

Result language

angličtina

Original language name

In vitro activation of CMV-specific human CD8(+) T cells by adenylate cyclase toxoids delivering pp65 epitopes

Original language description

Human CMV infects between 50-85% of healthy individuals and can cause live-threatening infections in immunocompromised patients. Therefore, peptide vaccination is being developed as a promising immunotherapeutic approach for treatment of patients at riskof CMV disease. The enzymatically inactive toxoid of Bordetella adenylate cyclase was shown to be an efficient tool for delivery of peptide epitopes and stimulation of Ag-specific T-cell immune responses. We investigated here the capacity of two CyaA-ACconstructs to deliver epitopes derived from the CMV phosphoprotein pp65 for activation of human T cells in vitro. Expansion of IFN-secreting CMV-specific CD8(+) T cells, as well as increase of total production by PBMCs from CMV-seropositive donors wereobserved after in vitro stimulation with CyaA-AC( constructs carrying CMV epitopes, whereas limited activation of immune response occurred with free peptides. The activation of immune response was confirmed by expansion of CMV-specific T-

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FD - Oncology and haematology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Bone Marrow Transplantation

ISSN

0268-3369

e-ISSN

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Volume of the periodical

47

Issue of the periodical within the volume

1

Country of publishing house

US - UNITED STATES

Number of pages

8

Pages from-to

243-250

UT code for WoS article

000300415100013

EID of the result in the Scopus database

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