In vitro activation of CMV-specific human CD8ţ T cells by adenylate

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F12%3A00377401" target="_blank" >RIV/61388971:_____/12:00377401 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1038/bmt.2011.68" target="_blank" >http://dx.doi.org/10.1038/bmt.2011.68</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/bmt.2011.68" target="_blank" >10.1038/bmt.2011.68</a>

Result language

angličtina

Original language name

In vitro activation of CMV-specific human CD8ţ T cells by adenylate

Original language description

Human CMV infects between 50-85% of healthy individuals and can cause live-threatening infections in immunocompromised patients. Therefore, peptide vaccination is being developed as a promising immunotherapeutic approach for treatment of patients at riskof CMV disease. The enzymatically inactive toxoid of Bordetella adenylate cyclase (CyaA-AC(-)) was shown to be an efficient tool for delivery of peptide epitopes and stimulation of Ag-specific T-cell immune responses. We investigated here the capacity of two CyaA-AC(-) constructs to deliver epitopes derived from the CMV phosphoprotein pp65 for activation of human T cells in vitro. Expansion of gamma-IFN-secreting CMV-specific CD8(+) T cells, as well as increase of total IFN-gamma and TNF-alpha production by PBMCs from CMV-seropositive donors were observed after in vitro stimulation with CyaA-AC(-) constructs carrying CMV epitopes, whereas limited activation of immune response occurred with free peptides

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Bone Marrow Transplantation

ISSN

0268-3369

e-ISSN

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Volume of the periodical

47

Issue of the periodical within the volume

2

Country of publishing house

GB - UNITED KINGDOM

Number of pages

8

Pages from-to

243-250

UT code for WoS article

000300415100013

EID of the result in the Scopus database

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