Roscovitine-induced apoptosis of H1299 cells depends on functional status of p53

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F65269705%3A_____%2F12%3A%230001897" target="_blank" >RIV/65269705:_____/12:#0001897 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14310/12:00064886

Result on the web

<a href="http://dx.doi.org/10.4149/neo_2012_077" target="_blank" >http://dx.doi.org/10.4149/neo_2012_077</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.4149/neo_2012_077" target="_blank" >10.4149/neo_2012_077</a>

Result language

angličtina

Original language name

Roscovitine-induced apoptosis of H1299 cells depends on functional status of p53

Original language description

Roscovitine, an inhibitor of cyclin-dependent kinases, is promising anticancer agent. Its antiproliferative and cytotoxic effects can be mediated by the p53 signaling pathway. To define the role of p53 in roscovitine-induced cell response, we prepared H1299/p53 cell lines inducibly expressing specific variants of p53 (p53wt and hotspot R175H, temperature-dependent P98A, A159V, S215G, Y220C, Y234C mutants). In the presence of roscovitine, each cell line variant behaved in specific way reflecting activityof the p53 protein. Roscovitine decreased production of the cell cycle inhibitor p21 and induced apoptosis. This effect was the most efficient in cells expressing p53wt protein with full activity. The cell expressing partially and conditionally active p53 mutants responded to roscovitine less efficiently. The cells expressing p53 mutants A159V and Y234C were very sensitive to roscovitine but their response was clearly temperature-dependent. The cells expressing P98A, S215G and Y220C p53

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FD - Oncology and haematology

OECD FORD branch

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Project

<a href="/en/project/NS10448" target="_blank" >NS10448: Detailed analyses of temperature-dependent mutants of the p53 tumor suppressor in human cells</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Neoplasma

ISSN

0028-2685

e-ISSN

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Volume of the periodical

59

Issue of the periodical within the volume

6

Country of publishing house

SK - SLOVAKIA

Number of pages

7

Pages from-to

606-612

UT code for WoS article

000310820200002

EID of the result in the Scopus database

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