Time- and Dose-Dependent Effects of 17 Beta-Estradiol on Short-Term, Real-Time Proliferation and Gene Expression in Porcine Granulosa Cells
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F65269705%3A_____%2F17%3A00066872" target="_blank" >RIV/65269705:_____/17:00066872 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14110/17:00096491
Result on the web
<a href="https://www.hindawi.com/journals/bmri/2017/9738640/" target="_blank" >https://www.hindawi.com/journals/bmri/2017/9738640/</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1155/2017/9738640" target="_blank" >10.1155/2017/9738640</a>
Alternative languages
Result language
angličtina
Original language name
Time- and Dose-Dependent Effects of 17 Beta-Estradiol on Short-Term, Real-Time Proliferation and Gene Expression in Porcine Granulosa Cells
Original language description
The key mechanisms responsible for achievement of full reproductive and developmental capability in mammals are the differentiation and transformation of granulosa cells (GCs) during folliculogenesis, oogenesis, and oocytematuration. Although the role of 17 beta-estradiol (E2) in ovarian activity is widely known, its effect on proliferative capacity, gap junction connection (GJC) formation, andGCs-luteal cells transformation requires further research. Therefore, the goal of this study was to assess the real-time proliferative activity of porcine GCs in vitro in relation to connexin (Cx), luteinizing hormone receptor (LHR), follicle stimulating hormone receptor (FSHR), and aromatase (CYP19A1) expression during short-term(168 h) primary culture. The cultured GCs were exposed to acute (at 96 h of culture) and/or prolonged (between 0 and 168 h of culture) administration of 1.8 and 3.6.. mu M E2. The relative abundance of Cx36, Cx37, Cx40, Cx43, LHR, FSHR, and CYP19A1mRNA wasmeasured. We conclude that the proliferation capability of GCs in vitro is substantially associated with expression of Cxs, LHR, FSHR, andCYP19A1. Furthermore, the GC-luteal cell transformation in vitro may be significantly accompanied by the proliferative activity of GCs in pigs.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
20802 - Bioremediation, diagnostic biotechnologies (DNA chips and biosensing devices) in environmental management
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
BioMed Research International
ISSN
2314-6133
e-ISSN
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Volume of the periodical
2017
Issue of the periodical within the volume
2017
Country of publishing house
US - UNITED STATES
Number of pages
9
Pages from-to
9738640
UT code for WoS article
000398229300001
EID of the result in the Scopus database
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