Size matters: Associations between the androgen receptor CAG repeat length and the intrafollicular hormone milieu

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064203%3A_____%2F16%3A10323708" target="_blank" >RIV/00064203:_____/16:10323708 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11130/16:10323708

Result on the web

<a href="http://dx.doi.org/10.1016/j.mce.2015.09.015" target="_blank" >http://dx.doi.org/10.1016/j.mce.2015.09.015</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.mce.2015.09.015" target="_blank" >10.1016/j.mce.2015.09.015</a>

Result language

angličtina

Original language name

Size matters: Associations between the androgen receptor CAG repeat length and the intrafollicular hormone milieu

Original language description

Granulosa cell (GC) expressed androgen receptors (AR) and intrafollicular androgens are central to fertility. The transactivating domain of the AR contains a polymorphic CAG repeat sequence, which is linked to the transcriptional activity of AR and may influence the GC function. This study aims to evaluate the effects of the AR CAG repeat length on the intrafollicular hormone profiles, and the gene expression profiles of GC from human small antral follicles. In total, 190 small antral follicles (3-11 mm in diameter) were collected from 58 women undergoing ovarian cryopreservation for fertility preservation. The biallelic mean of the CAG repeat lengths were calculated for each woman, and grouped in three groups: Long CAG repeats (23-26 mean CAG); medium CAG repeats (20.5-22.5 mean CAG) and short CAG repeats (17.5-20.0 mean CAG). The following parameters were measured: follicle diameter, intrafollicular levels of Anti-Mtillerian Hormone (AMH), progesterone, oestradiol, testosterone and androstenedione, and GC gene expression levels of FSHR, LHR, AR, CYP19A1, and AMH. The long CAG repeat lengths were associated with significantly decreased testosterone levels, as compared to medium CAG repeats (P = 0.05) and short CAG repeats (P = 0.003). Furthermore, in follicles 3-6 mm in diameter, the long CAG repeats were associated with significantly increased LHR and CYP19A1 gene expression levels compared to short CAG repeat lengths (P = 0.004 and P = 0.04 respectively), and significantly increased LHR expression compared to medium CAG repeat lengths (P = 0.03). In conclusion, long CAG repeat lengths in the AR were associated to significant attenuated levels of androgens and an increased conversion of testosterone into oestradiol, in human small antral follicles.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/NT13770" target="_blank" >NT13770: Examination of DNA instability in gametes, embryos and somatic cells following oncological therapy for improvement of reproductive success in treated patients.</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Molecular and Cellular Endocrinology

ISSN

0303-7207

e-ISSN

—

Volume of the periodical

419

Issue of the periodical within the volume

C

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

6

Pages from-to

12-17

UT code for WoS article

000370991700002

EID of the result in the Scopus database

2-s2.0-84949575373