Diversity of Pneumocystis jirovecii Across Europe: A Multicentre Observational Study

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F65269705%3A_____%2F17%3A00067342" target="_blank" >RIV/65269705:_____/17:00067342 - isvavai.cz</a>

Result on the web

<a href="https://ac.els-cdn.com/S2352396417302621/1-s2.0-S2352396417302621-main.pdf?_tid=ccaaeae6-cf85-11e7-81dc-00000aacb35e&acdnat=1511356103_0d6795e012f542d5d95df5b91b692c6f" target="_blank" >https://ac.els-cdn.com/S2352396417302621/1-s2.0-S2352396417302621-main.pdf?_tid=ccaaeae6-cf85-11e7-81dc-00000aacb35e&acdnat=1511356103_0d6795e012f542d5d95df5b91b692c6f</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ebiom.2017.06.027" target="_blank" >10.1016/j.ebiom.2017.06.027</a>

Result language

angličtina

Original language name

Diversity of Pneumocystis jirovecii Across Europe: A Multicentre Observational Study

Original language description

Pneumocystis jirovecii is an airborne human-specific ascomycetous fungus responsible for Pneumocystis pneumonia (PCP) in immunocompromised patients, affecting N500,000 patients per year (www.gaffi.org). The understanding of its epidemiology is limited by the lack of standardised culture. Recent genotyping data suggests a limited genetic diversity of P. jirovecii. The objective of the study was to assess the diversity of P. jirovecii across European hospitals and analyse P. jirovecii diversity in respect to clinical data obtained from the patients. Genotypingwas performed using six already validated short tandem repeat (STR) markers on 249 samples (median: 17 per centre interquartile range [11-20]) from PCP patients of 16 European centres. Mixtures of STR markers (i.e., &gt;= 2 alleles for &gt;= 1 locus) were detected in 67.6% (interquartile range [61.4; 76.5]) of the samples. Mixture was significantly associated with the underlying disease of the patient, with an increased proportion in HIV patients (78.3%) and a decreased proportion in renal transplant recipients (33.3%) (p &lt; 0.001). The distribution of the alleles was significantly different (p &lt; 0.001) according to the centres in three out of six markers. In analysable samples, 201 combinations were observed corresponding to 137 genotypes: 116 genotypes were country-specific; 12 in two; six in three; and two in four and one in five countries. Nine genotypes were recorded more than once in a given country. Genotype 123 (Gt123) was significantly associated with France (14/15, p &lt; 0.001) and Gt16 with Belgium (5/5, p &lt; 0.001). More specifically, Gt123 was observed mainly in France (14/15/16 patients) and in renal transplant patient (13/15). Our study showed the wide population diversity across Europe, with evidence of local clusters of patients harbouring a given genotype.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

30302 - Epidemiology

Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

EBioMedicine

ISSN

2352-3964

e-ISSN

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Volume of the periodical

22

Issue of the periodical within the volume

AUG 2017

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

9

Pages from-to

155-163

UT code for WoS article

000410738600020

EID of the result in the Scopus database

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