Magnetic-Activated Cell Sorting in Combination with Swim-Up Efficiency Improve Effectivity of Spermatozoa Separation

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F65269705%3A_____%2F18%3A00069402" target="_blank" >RIV/65269705:_____/18:00069402 - isvavai.cz</a>
Result on the web
<a href="https://content.sciendo.com/view/journals/acb/6/2/article-p55.xml" target="_blank" >https://content.sciendo.com/view/journals/acb/6/2/article-p55.xml</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.2478/acb-2018-0010" target="_blank" >10.2478/acb-2018-0010</a>

Result language
angličtina
Original language name
Magnetic-Activated Cell Sorting in Combination with Swim-Up Efficiency Improve Effectivity of Spermatozoa Separation
Original language description
Correct selection of spermatozoa before their using for an assisted reproductive techniques is one of the crucial step in therapy of human infertility. It was previously reported that male factor plays a major role in infertility. Basic semen analyses and standard methods for sperm selection in many cases does not eliminate sufficiently proportion of spermatozoa with genetics defects. Magnetic Activated Cell Sorting (MACS) is a selection method which reduce apoptotic sperm and improve sperm and embryo quality. The aim of our study was the comparison of swim-up method and MACS and their combination. We tested swim-up and MACS alone and treatment of spermatozoa in combination when was is first swim-up and second MACS and vice versa. In this study we evaluated sperm concentration, motility and their DNA integrity before and after separtion methods. On the basis of our results we recommend to use swim-up before MACS method. This approach brings better results in the sperm selection - lower proportion of spermatozoa with fragmented DNA and also it brings better gain of total spermatozoa usable for next IVF or ICSI methods.
Czech name
—
Czech description
—

Type
J<sub>SC</sub> - Article in a specialist periodical, which is included in the SCOPUS database
CEP classification
—
OECD FORD branch
30214 - Obstetrics and gynaecology

Project
<a href="/en/project/LTC18059" target="_blank" >LTC18059: Cultivation system suitable for long-term cultivation of human follicular cells</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Similar results(10)