Novel markers of human ovarian granulosa cell differentiation toward osteoblast lineage: A microarray approach

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F65269705%3A_____%2F19%3A00072306" target="_blank" >RIV/65269705:_____/19:00072306 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14110/19:00112986

Result on the web

<a href="https://www.spandidos-publications.com/mmr/20/5/4403" target="_blank" >https://www.spandidos-publications.com/mmr/20/5/4403</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3892/mmr.2019.10709" target="_blank" >10.3892/mmr.2019.10709</a>

Result language

angličtina

Original language name

Novel markers of human ovarian granulosa cell differentiation toward osteoblast lineage: A microarray approach

Original language description

Under physiological conditions, human ovarian granulosa cells (GCs), are responsible for a number of processes associated with folliculogenesis and oogenesis. The primary functions of GCs in the individual phases of follicle growth are: Hormone production in response to follicle stimulating hormone (FSH), induction of ovarian follicle atresia through specific molecular markers and production of nexus cellular connections for communication with the oocyte. In recent years, interest in obtaining stem cells from particular tissues, including the ovary, has increased. Special attention has been paid to the novel properties of GCs during long-term in vitro culture. It has been demonstrated that the usually recycled material in the form of follicular fluid can be a source of cells with stem-like properties. The study group consisted of patients enrolled in the in vitro fertilization procedure. Total RNA was isolated from GCs at 4 time points (after 1, 7, 15 and 30 days of culture) and was used for microarray expression analysis (Affymetrix (R) Human HgU 219 Array). The expression of 22,480 transcripts was examined. The selection of significantly altered genes was based on a P-value &lt;0.05 and expression higher than two-fold. The leucine rich repeat containing 17, collagen type I alpha 1 chain, bone morphogenetic protein 4, twist family bHLH transcription factor 1, insulin like growth factor binding protein 5, GLI family zinc finger 2 and collagen triple helix repeat containing genes exhibited the highest changes in expression. Reverse-transcription-quantitative PCR was performed to validate the results obtained in the analysis of expression microarrays. The direction of expression changes was validated in the majority of cases. The presented results indicated that GCs have the potential of cells that can differentiate towards osteoblasts in long-term in vitro culture conditions. Increased expression of genes associated with the osteogenesis process suggests a potential for uninduced change of GC properties towards the osteoblast phenotype. The present study, therefore, suggests that GCs may become an excellent starting material in obtaining stable osteoblast cultures. GCs differentiated towards osteoblasts may be used in regenerative and reconstructive medicine in the future.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

30100 - Basic medicine

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Molecular Medicine Reports

ISSN

1791-2997

e-ISSN

—

Volume of the periodical

20

Issue of the periodical within the volume

5

Country of publishing house

GR - GREECE

Number of pages

12

Pages from-to

4403-4414

UT code for WoS article

000491393100046

EID of the result in the Scopus database

2-s2.0-85073116401