Specificity of 3D MSC Spheroids Microenvironment: Impact on MSC Behavior and Properties

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F65269705%3A_____%2F20%3A00073022" target="_blank" >RIV/65269705:_____/20:00073022 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14110/20:00116197

Result on the web

<a href="https://link.springer.com/article/10.1007/s12015-020-10006-9" target="_blank" >https://link.springer.com/article/10.1007/s12015-020-10006-9</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s12015-020-10006-9" target="_blank" >10.1007/s12015-020-10006-9</a>

Result language

angličtina

Original language name

Specificity of 3D MSC Spheroids Microenvironment: Impact on MSC Behavior and Properties

Original language description

Mesenchymal stem cells (MSC) have been considered the promising candidates for the regenerative and personalized medicine due to their self-renewal potential, multilineage differentiation and immunomodulatory capacity. Although these properties have encouraged profound MSC studies in recent years, the majority of research has been based on standard 2D culture utilization. The opportunity to resemble in vivo characteristics of cells native niche has been provided by implementation of 3D culturing models such as MSC spheroid formation assesed through cells self-assembling. In this review, we address the current literature on physical and biochemical features of 3D MSC spheroid microenvironment and their impact on MSC properties and behaviors. Starting with the reduction in the cells&apos; dimensions and volume due to the changes in adhesion molecules expression and cytoskeletal proteins rearrangement resembling native conditions, through the microenvironment shifts in oxygen, nutrients and metabolites gradients and demands, we focus on distinctive and beneficial features of MSC in spheroids compared to cells cultured in 2D conditions. By summarizing the data for 3D MSC spheroids regarding cell survival, pluripotency, differentiation, immunomodulatory activities and potential to affect tumor cells growth we highlighted advantages and perspectives of MSC spheroids use in regenerative medicine. Further detailed analyses are needed to deepen our understanding of mechanisms responsible for modified MSC behavior in spheroids and to set future directions for MSC clinical application.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10601 - Cell biology

Project

<a href="/en/project/LTC18059" target="_blank" >LTC18059: Cultivation system suitable for long-term cultivation of human follicular cells</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Stem Cell Reviews and Reports

ISSN

2629-3269

e-ISSN

—

Volume of the periodical

16

Issue of the periodical within the volume

5

Country of publishing house

US - UNITED STATES

Number of pages

23

Pages from-to

853-875

UT code for WoS article

000549663600001

EID of the result in the Scopus database

2-s2.0-85088108884