Capture-based NGS panel for detection of immunoglobulin and T-cell receptor gene rearrangements in DNA samples from patients with acute lymphoblastic leukemia
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F65269705%3A_____%2F20%3A00073230" target="_blank" >RIV/65269705:_____/20:00073230 - isvavai.cz</a>
Result on the web
<a href="https://journals.lww.com/hemasphere/Citation/2020/06001/Abstract_Book__25th_Congress_of_the_European.1.aspx" target="_blank" >https://journals.lww.com/hemasphere/Citation/2020/06001/Abstract_Book__25th_Congress_of_the_European.1.aspx</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1097/HS9.0000000000000404" target="_blank" >10.1097/HS9.0000000000000404</a>
Alternative languages
Result language
angličtina
Original language name
Capture-based NGS panel for detection of immunoglobulin and T-cell receptor gene rearrangements in DNA samples from patients with acute lymphoblastic leukemia
Original language description
Background: Monitoring of minimal residual disease (MRD) is a strong prognostic factor of clinical outcome in ALL patients. There are several different techniques available for MRD detection. Real-time quantitative PCR (RT-qPCR) with patient-specific primers represents a method well standardized by EuroMRD Working Group, which is still widely used despite great advances in next-generation sequencing. Acquiring of individual immunoglobulin (IG)/T-cell receptor (TR) junctional region sequences for primer design using traditional Sanger sequencing is often difficult due to the presence of polyclonal background and due to the necessity of multiplex PCR usage for IG/TR gene amplification. Aims: Our aim was to assess applicability of our recently established versatile NGS panel for characterization of clonal IG/TR rearrangements in ALL samples.
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
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OECD FORD branch
30205 - Hematology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů