Role of miR-653 and miR-29c in downregulation of CYP1A2 expression in hepatocellular carcinoma
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F65269705%3A_____%2F22%3A00075993" target="_blank" >RIV/65269705:_____/22:00075993 - isvavai.cz</a>
Alternative codes found
RIV/68081707:_____/22:00555407 RIV/00216224:14310/22:00125067 RIV/00179906:_____/22:10449864 RIV/00209805:_____/22:00078890
Result on the web
<a href="https://link.springer.com/article/10.1007/s43440-021-00338-9" target="_blank" >https://link.springer.com/article/10.1007/s43440-021-00338-9</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s43440-021-00338-9" target="_blank" >10.1007/s43440-021-00338-9</a>
Alternative languages
Result language
angličtina
Original language name
Role of miR-653 and miR-29c in downregulation of CYP1A2 expression in hepatocellular carcinoma
Original language description
Background Hepatocellular carcinoma (HCC) is a major contributor to the worldwide cancer burden. Recent studies on HCC have demonstrated dramatic alterations in expression of several cytochrome P450 (CYP) family members that play a crucial role in biotransformation of many drugs and other xenobiotics; however, the mechanisms responsible for their deregulation remain unclear. Methods We investigated a potential involvement of miRNAs in downregulation of expression of CYPs observed in HCC tumors. We compared miRNA expression profiles (TaqMan Array Human MicroRNA v3.0 TLDA qPCR) between HCC human patient tumors with strong (CYP-) and weak/no (CYP+) downregulation of drug-metabolizing CYPs. The role of significantly deregulated miRNAs in modulation of expression of the CYPs and associated xenobiotic receptors was then investigated in human liver HepaRG cells transfected with relevant miRNA mimics or inhibitors. Results We identified five differentially expressed miRNAs in CYP- versus CYP+ tumors, namely miR-29c, miR-125b1, miR-505, miR-653 and miR-675. The two most-upregulated miRNAs found in CYP- tumor samples, miR-29c and miR-653, were found to act as efficient suppressors of CYP1A2 or AHR expression. Conclusions Our results revealed a novel role of miR-653 and miR-29c in regulation of expresion of CYPs involved in crucial biotransformation processes in liver, which are often deregulated during liver cancer progression. [GRAPHICS] .
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30100 - Basic medicine
Result continuities
Project
<a href="/en/project/NV17-28231A" target="_blank" >NV17-28231A: Expression of biotransformation enzymes in primary liver cancers</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Pharmacological Reports
ISSN
1734-1140
e-ISSN
2299-5684
Volume of the periodical
74
Issue of the periodical within the volume
1
Country of publishing house
DE - GERMANY
Number of pages
11
Pages from-to
148-158
UT code for WoS article
000718694600001
EID of the result in the Scopus database
2-s2.0-85119055842