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Effective NPM1 plasmid standards selection for minimal/measurable residual disease monitoring in acute myeloid leukemia

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F65269705%3A_____%2F22%3A00076175" target="_blank" >RIV/65269705:_____/22:00076175 - isvavai.cz</a>

  • Alternative codes found

    RIV/00216224:14110/22:00126108

  • Result on the web

    <a href="https://link.springer.com/article/10.1007/s11033-022-07363-8" target="_blank" >https://link.springer.com/article/10.1007/s11033-022-07363-8</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1007/s11033-022-07363-8" target="_blank" >10.1007/s11033-022-07363-8</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Effective NPM1 plasmid standards selection for minimal/measurable residual disease monitoring in acute myeloid leukemia

  • Original language description

    Background NPM1 plasmid standards are required for absolute quantification of minimal residual disease in acute myeloid leukemia patients. The standards are usually obtained, next to commercially constructed gene fragments, from transgenic bacteria colonies. However, this procedure is laborious and very time consuming. Methods and results We have developed a PCR method that speeds up, simplifies, and streamlines the process of preparing NPM1 plasmid standards. The method is based on a combination of three primers, two surrounding the usual NPM1 mutation position and one over the mutation site. With this method, we were able to clearly distinguish plasmids with at least 15 different NPM1 mutations from the wild-type NPM1 plasmid. Conclusions With the new approach, preparing NPM1 plasmid standards is easier, identifying NPM1-positive colonies is possible in less than a day and moreover, for a lower price than commercially constructed gene fragments.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10608 - Biochemistry and molecular biology

Result continuities

  • Project

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2022

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Molecular Biology Reports

  • ISSN

    0301-4851

  • e-ISSN

    1573-4978

  • Volume of the periodical

    49

  • Issue of the periodical within the volume

    8

  • Country of publishing house

    NL - THE KINGDOM OF THE NETHERLANDS

  • Number of pages

    4

  • Pages from-to

    8169-8172

  • UT code for WoS article

    000812606600010

  • EID of the result in the Scopus database

    2-s2.0-85132138417