Effective NPM1 plasmid standards selection for minimal/measurable residual disease monitoring in acute myeloid leukemia
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F65269705%3A_____%2F22%3A00076175" target="_blank" >RIV/65269705:_____/22:00076175 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14110/22:00126108
Result on the web
<a href="https://link.springer.com/article/10.1007/s11033-022-07363-8" target="_blank" >https://link.springer.com/article/10.1007/s11033-022-07363-8</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s11033-022-07363-8" target="_blank" >10.1007/s11033-022-07363-8</a>
Alternative languages
Result language
angličtina
Original language name
Effective NPM1 plasmid standards selection for minimal/measurable residual disease monitoring in acute myeloid leukemia
Original language description
Background NPM1 plasmid standards are required for absolute quantification of minimal residual disease in acute myeloid leukemia patients. The standards are usually obtained, next to commercially constructed gene fragments, from transgenic bacteria colonies. However, this procedure is laborious and very time consuming. Methods and results We have developed a PCR method that speeds up, simplifies, and streamlines the process of preparing NPM1 plasmid standards. The method is based on a combination of three primers, two surrounding the usual NPM1 mutation position and one over the mutation site. With this method, we were able to clearly distinguish plasmids with at least 15 different NPM1 mutations from the wild-type NPM1 plasmid. Conclusions With the new approach, preparing NPM1 plasmid standards is easier, identifying NPM1-positive colonies is possible in less than a day and moreover, for a lower price than commercially constructed gene fragments.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
—
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Molecular Biology Reports
ISSN
0301-4851
e-ISSN
1573-4978
Volume of the periodical
49
Issue of the periodical within the volume
8
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
4
Pages from-to
8169-8172
UT code for WoS article
000812606600010
EID of the result in the Scopus database
2-s2.0-85132138417