Magnetic particles?based biosensor for biogenic amines using an optical oxygen sensor as a transducer

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67179843%3A_____%2F13%3A00388384" target="_blank" >RIV/67179843:_____/13:00388384 - isvavai.cz</a>

Alternative codes found

RIV/67985858:_____/13:00388384 RIV/61989592:15310/13:33147699

Result on the web

<a href="http://dx.doi.org/10.1007/s00604-012-0932-0" target="_blank" >http://dx.doi.org/10.1007/s00604-012-0932-0</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00604-012-0932-0" target="_blank" >10.1007/s00604-012-0932-0</a>

Result language

angličtina

Original language name

Magnetic particles?based biosensor for biogenic amines using an optical oxygen sensor as a transducer

Original language description

We have developed a fibre optic biosensor with incorporated magnetic microparticles for the determination of biogenic amines. The enzyme diamine oxidase from Pisum sativum was immobilized either on chitosan-coated magnetic microparticles or on commercialmicrobeads modified with a ferrofluid. Both the immobilized enzyme and the ruthenium complex were incorporated into a UV-cured inorganic?organic polymer composite and deposited on a lens that was connected, by optical fibres, to an electro-optical detector. The enzyme catalyzes the oxidation of amines under consumption of oxygen. The latter was determined by measuring the quenched fluorescence lifetime of the ruthenium complex. The limits of detection for the biogenic amines putrescine and cadaverine are 25?30 ?mol?L1, and responses are linear up to a concentration of 1 mmol L1.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EH - Ecology - communities

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Microchimica Acta

ISSN

0026-3672

e-ISSN

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Volume of the periodical

180

Issue of the periodical within the volume

3-4

Country of publishing house

AT - AUSTRIA

Number of pages

8

Pages from-to

311-318

UT code for WoS article

000313837900017

EID of the result in the Scopus database

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