Development of methods for breeding high-lipidcontent algal strain Chlamydomonas reinhardtii using fluorescence-activated cell sorting
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67179843%3A_____%2F15%3A00456345" target="_blank" >RIV/67179843:_____/15:00456345 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Development of methods for breeding high-lipidcontent algal strain Chlamydomonas reinhardtii using fluorescence-activated cell sorting
Original language description
Green microalgae are among the most widely distributed microorganisms in the biosphere. They are significant contributors to global photosynthetic productivity and are interesting for biotechnology due to their large variety of high-value compound accumulation and range of applications. To achieve profitable microalgae cultures for biotechnology, one wants to combine antagonistic properties: rapid growth and high accumulation of specific compounds. Here, we focus on development of advanced cultivation strategies and breeding methods applied to the model algae Chlamydomonas reinhardtii for optimized production of lipids. For identification, isolation, and subsequent selection of an optimal subpopulation with high lipid content, we used high-throughput fluorescenceactivated cell sorting in combination with imaging flow cytometry on cells stained with lipid-specific fluorescent dye. We observed that post-sort cell viability was not negatively influenced by external parameters used during
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
EH - Ecology - communities
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
Global Change: A Complex Challenge : Conference Proceedings
ISBN
978-80-87902-10-3
ISSN
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e-ISSN
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Number of pages
4
Pages from-to
150-153
Publisher name
Global Change Research Centre, The Czech Academy of Sciences, v. v. i.
Place of publication
Brno
Event location
Brno
Event date
Mar 23, 2015
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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