Raman microspectroscopy of algal lipid bodies: ?-carotene as a volume sensor
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081731%3A_____%2F11%3A00368584" target="_blank" >RIV/68081731:_____/11:00368584 - isvavai.cz</a>
Alternative codes found
RIV/60646594:_____/11:#0000013
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Raman microspectroscopy of algal lipid bodies: ?-carotene as a volume sensor
Original language description
Advanced optical instruments are useful for analysis and manipulation of individual living cells and their internal structures. We have employed Raman microspectroscopic analysis for assessment of algal lipid body (LB) volume in vivo. Some algae contain?-carotene in high amounts in their LBs, including strains which are considered useful in biotechnology for lipid and pigment production. We have detected proportionality between the Raman vibrations of ?-carotene and the LB volume. This finding may allow fast acquisition of LB volume approximation valuable e.g. for Raman microspectroscopy assisted cell sorting. We combine optical manipulation and analysis on a microfluidic platform in order to achieve fast, effective, and non-invasive sorting based onspectroscopic features of the individual living cells. The resultant apparatus could find its use in demanding biotechnological applications such as selection of rare natural mutants or artificially modified cells resulting from genetic m
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
BH - Optics, masers and lasers
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
Photonics, Devices, and Systems V (Proceedings of SPIE Vol. 8306)
ISBN
978-0-8194-8953-1
ISSN
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e-ISSN
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Number of pages
7
Pages from-to
"83060L:1"-"7"
Publisher name
SPIE
Place of publication
Bellingham
Event location
Praha
Event date
Aug 24, 2011
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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