Raman microspectroscopy of algal lipid bodies: beta-carotene quantification

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081731%3A_____%2F12%3A00383732" target="_blank" >RIV/68081731:_____/12:00383732 - isvavai.cz</a>

Alternative codes found

RIV/67179843:_____/12:00383732 RIV/60646594:_____/12:#0000008

Result on the web

<a href="http://dx.doi.org/10.1007/s10811-011-9754-4" target="_blank" >http://dx.doi.org/10.1007/s10811-011-9754-4</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10811-011-9754-4" target="_blank" >10.1007/s10811-011-9754-4</a>

Result language

angličtina

Original language name

Raman microspectroscopy of algal lipid bodies: beta-carotene quantification

Original language description

Advanced optical instruments can serve for analysis and manipulation of individual living cells and their internal structures. We have used Raman microspectroscopic analysis for assessment of beta-carotene concentration in algal lipid bodies (LBs) in vivo. Some algae contain beta-carotene in high amounts in their LBs, including strains which are considered useful in biotechnology for lipid and pigment production. We have devised a simple method to measure the concentration of beta-carotene in a mixtureof algal storage lipids from the ratio of their Raman vibrations. This finding may allow fast acquisition of beta-carotene concentration valuable, e.g., for Raman microspectroscopy assisted cell sorting for selection of the overproducing strains. Furthermore, we demonstrate that beta-carotene concentration can be proportional to LB volume and light intensity during the cultivation. We combine optical manipulation and analysis on a microfluidic platform in order to achieve fast, effective

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

JA - Electronics and optoelectronics

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Applied Phycology

ISSN

0921-8971

e-ISSN

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Volume of the periodical

24

Issue of the periodical within the volume

3

Country of publishing house

US - UNITED STATES

Number of pages

6

Pages from-to

541-546

UT code for WoS article

000304187100029

EID of the result in the Scopus database

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