Raman tweezers in microfluidic systems for analysis and sorting of living cells

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081731%3A_____%2F14%3A00437742" target="_blank" >RIV/68081731:_____/14:00437742 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1117/12.2176047" target="_blank" >http://dx.doi.org/10.1117/12.2176047</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1117/12.2176047" target="_blank" >10.1117/12.2176047</a>

Result language

angličtina

Original language name

Raman tweezers in microfluidic systems for analysis and sorting of living cells

Original language description

We have devised an analytical and sorting system combining optical trapping with Raman spectroscopy in microfluidic environment, dedicated to identification and sorting of biological objects, such as living cells of various unicellular organisms. Our main goal was to create a robust and universal platform for non-destructive and non-contact sorting of micro-objects based on their Raman spectral properties. This approach allowed us to collect spectra containing information about the chemical compositionof the objects, such as the presence and composition of pigments, lipids, proteins, or nucleic acids, avoiding artificial chemical probes such as fluorescent markers. The non-destructive nature of this optical analysis and manipulation allowed us to separate individual living cells of our interest in a sterile environment and provided the possibility to cultivate the selected cells for further experiments. We used a mixture of polystyrene micro-particles and algal cells to test and demon

Czech name

—

Czech description

—

Type

D - Article in proceedings

CEP classification

BH - Optics, masers and lasers

OECD FORD branch

—

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

19th Polish-Slovak-Czech Optical Conference on Wave and Quantum Aspects of Contemporary Optics (Proceedings of SPIE 9441)

ISBN

9781628415568

ISSN

0277-786X

e-ISSN

—

Number of pages

8

Pages from-to

"944107:1"-"8"

Publisher name

SPIE

Place of publication

Bellingham

Event location

Jelenia Góra

Event date

Sep 8, 2014

Type of event by nationality

EUR - Evropská akce

UT code for WoS article

—