Raman tweezers in microfluidic systems for automatic analysis and sorting of living cells
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081731%3A_____%2F14%3A00436456" target="_blank" >RIV/68081731:_____/14:00436456 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Raman tweezers in microfluidic systems for automatic analysis and sorting of living cells
Original language description
We have devised an automatic analytical and sorting system combining optical trapping with Raman spectroscopy in microfluidic environment, together with computerized real time image analysis, spectra processing and micromanipulation. This device serves to identify and sort biological objects, such as living cells of various prokaryotic and eukaryotic organisms based on their Raman spectral properties. This approach allowed us to collect information about the chemical composition of the objects, such asthe presence and composition of lipids, proteins, or nucleic acids without using artificial chemical probes such as fluorescent markers. The non-destructive and non-contact nature of this optical analysis and manipulation allowed us to separate individual living cells of our interest in a sterile environment and provided the possibility to cultivate the selected cells for further experiments. The special microfluidic chip uses gravity to move the cells across the sorting area. Our system
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
BH - Optics, masers and lasers
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
Sborník příspěvků multioborové konference Laser54
ISBN
978-80-87441-13-8
ISSN
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e-ISSN
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Number of pages
1
Pages from-to
60
Publisher name
Ústav přístrojové techniky AV ČR
Place of publication
Brno
Event location
Třešť
Event date
Oct 29, 2014
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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