Raman tweezers in microfluidic systems for analysis and sorting of living cells
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081731%3A_____%2F14%3A00434961" target="_blank" >RIV/68081731:_____/14:00434961 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1117/12.2040631" target="_blank" >http://dx.doi.org/10.1117/12.2040631</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1117/12.2040631" target="_blank" >10.1117/12.2040631</a>
Alternative languages
Result language
angličtina
Original language name
Raman tweezers in microfluidic systems for analysis and sorting of living cells
Original language description
We have devised an analytical and sorting system combining optical trapping with Raman spectroscopy in microfluidic environment in order to identify and sort biological objects, such as living cells of various prokaryotic and eukaryotic organisms. Our main objective was to create a robust and universal platform for non-contact sorting of microobjects based on their Raman spectral properties. This approach allowed us to collect information about the chemical composition of the objects, such as the presence and composition of lipids, proteins, or nucleic acids without using artificial chemical probes such as fluorescent markers. The non-destructive and non-contact nature of this optical analysis and manipulation allowed us to separate individual living cells of our interest in a sterile environment and provided the possibility to cultivate the selected cells for further experiments. We used differently treated cells of algae to test and demonstrate the function of our analytical and sort
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
BH - Optics, masers and lasers
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII (Proceedings of SPIE 8947)
ISBN
9780819498601
ISSN
1605-7422
e-ISSN
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Number of pages
9
Pages from-to
"89471M:1"-"9"
Publisher name
SPIE
Place of publication
Bellingham
Event location
San Francisco
Event date
Feb 3, 2014
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
000336037200037