Transport kinetics of uncoupling proteins: analysis of UCP1 reconstituted in planar lipid bilayers.

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F03%3A20030099" target="_blank" >RIV/67985823:_____/03:20030099 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Transport kinetics of uncoupling proteins: analysis of UCP1 reconstituted in planar lipid bilayers.
Original language description
According to alternative hypotheses, mitochondrial uncoupling protein 1 (UCP1) is either a proton channel (buffering model) or a fatty acid anion carrier (fatty acid cycling). Transport across the proton channel along a chain of hydrogen bonds (Grotthusmechanism) may include fatty acid carboxyl groups or occur in the absence of fatty acids. In the present work we demonstrate that planar bilayers reconstituted with UCP1 exhibit an increase in membrane conductivity exclusively in the presence of fatty acids (FA). Hence we can exclude the hypothesis, considering a pre-existing H+ channel in UCP1 which does not require FA for function. The augmented conductivity is nearly completely blocked by ATP. Direct application of transmembrane voltage and precise current measurements allowed determination of ATP-sensitive conductances at 0 and 150 mV as 11.5 pS and 54.3 pS, respectively, by reconstituting nearly 3*105 copies of UCP1. The proton conductivity measurements carried out in presence of a
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
BO - Biophysics
OECD FORD branch
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Project
<a href="/en/project/IAA5011106" target="_blank" >IAA5011106: Studies of novel mitochondrial uncoupling proteins</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2003
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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