Histogram warping based correction of the light attenuation with depth of data sets captured by a confocal microscope

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F05%3A00021202" target="_blank" >RIV/67985823:_____/05:00021202 - isvavai.cz</a>

Alternative codes found

RIV/68407700:21460/05:12109462

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Histogram warping based correction of the light attenuation with depth of data sets captured by a confocal microscope

Original language description

A confocal laser scanning microscope (CLSM) gives possibility to capture images from a biological specimen in different depths and obtain a stack of precisely registered fluorescent images, i.e. a 3D image. However, fluorescent image intensities from a CLSM suffer from light loss distortions. This effect causes problems in subsequent analysis of biological objects. Therefore, methods for compensation of the light attenuation with depth must be used. It is possible to solve this problem by a feedback-based tuning of the CLSM hardware during scanning, but in this paper we pay attention to post-processing methods and developed such one. It is a two step algorithm. In the first step the algorithm determines a standard histogram of the stack of images and in the second step histograms of individual images are warped according to this standard histogram. The algorithm results in more stable brightness and contrast of images through the stack

Czech name

Korekce útlumu jasu s hloubkou datových souborů získaných konfokálním mikroskopem založená na deformaci histogramu

Czech description

Obrazy získané konfokálním mikroskopem jsou zatížené útlumem jasu a kontrastu s hloubkou zaostření. Vyvinuli jsme metodu založenou na deformaci histogramů těchto obrazů, která tento problém eliminuje

Type

D - Article in proceedings

CEP classification

JD - Use of computers, robotics and its application

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2005

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Proceedings of the Fifth IASTED International Conference on Visualization, Imaging and Image Processing

ISBN

0-88986-528-0

ISSN

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e-ISSN

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Number of pages

6

Pages from-to

12-17

Publisher name

ACTA Press

Place of publication

Anaheim

Event location

Benidorm

Event date

Sep 7, 2005

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

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