Methods for compensation of the light attenuation with depth of images captured by a confocal microscope

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F06%3A00049753" target="_blank" >RIV/67985823:_____/06:00049753 - isvavai.cz</a>

Alternative codes found

RIV/68407700:21460/06:12120277

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Methods for compensation of the light attenuation with depth of images captured by a confocal microscope

Original language description

A confocal laser scanning microscope (CLSM) enables us to capture images from a biological specimen in different depths and obtain a series of precisely registered fluorescent images. But images captured from deep layers of the specimen may be darker than images from the topmost layers due to light loss distortions. This effect causes difficulties in subsequent analysis of biological objects. We propose a solution using two approaches: either an online method working already during image acquisition, oran offline method assisting as a post-processing step. In the online method the gain value of a photomultiplier tube of a CLSM is controlled according to the difference of mean image intensities between the reference and currently acquired image. The offline method consists of two stages. In the first stage a standard histogram improving brightness and contrast is created from all images in the series. In the second stage image histograms are warped according to this standard histogram

Czech name

Metody kompenzace útlumu jasu s hloubkou obrazů získaných konfokálním mikroskopem

Czech description

Konfokální laserový mikroskop umožňuje získat 3D obrazy biologického vzorku. Snímání je však zatíženo útlumem jasu a kontrastu obrazů s hloubkou zaostření. Článek popisuje metody kompenzace tohoto útlumu jednak během samotného snímaní a jednak úpravou obrazů po nasnímání

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

JC - Computer hardware and software

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2006

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Microscopy Research Technique

ISSN

1059-910X

e-ISSN

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Volume of the periodical

69

Issue of the periodical within the volume

8

Country of publishing house

US - UNITED STATES

Number of pages

12

Pages from-to

624-635

UT code for WoS article

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EID of the result in the Scopus database

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