Redistribution of cell death-inducing DNA fragmentation factor-like effecto-a (CIDE-a) from mitochondria to nucleus is associated with apoptosis in HeLa cells

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F08%3A00314551" target="_blank" >RIV/67985823:_____/08:00314551 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Redistribution of cell death-inducing DNA fragmentation factor-like effecto-a (CIDE-a) from mitochondria to nucleus is associated with apoptosis in HeLa cells

Original language description

CIDEa has been immunodetected in whole cells and subcellular fractions of HeLa cells adapted for a tetracycline-inducible CIDEa expression. Using immunocytochemistry we observed redistribution, enhanced upon treatment with camptothecin or valinomycin, ofCIDEa from mitochondria to nucleus. Similarly, CIDEa content increased in the nuclear fraction but decreased in cytosolic fraction in cells treated to initiate apoptosis. We hypothesize that CIDEa is sequestered in mitochondria while transfer of this potentially dangerous protein from mitochondria into nucleus intensifies or even initiates apoptosis

Czech name

Redistribuce faktoru podobného DNA fragmentačnímu faktoru indukujícímu buněčnou smrt mezi mitochondriemi a jádrem ja asociována s apoptózou v HeLa buňkách

Czech description

Protein CIDEa byl imunodetkecí lokalizován u subcelulárních frakcí HeLa buněk adaptovaných na tetracyklinem indukovanou expresi. Zjistili jsme redistribuci CIDEa z mitochondrií do jádra po přídavku kapmhotecinu a valinomycinu a u buněk s počínající apoptózou. Hypoteticky CIDEa sekvesterovaná v mitochondriích tak může exportem do jádra iniciovat apoptózu

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EA - Morphology and cytology

OECD FORD branch

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Project

<a href="/en/project/GA301%2F05%2F0221" target="_blank" >GA301/05/0221: Elucidation of role of mitochondrial factor CIDEa in normal physiology, antioxidant defense, and in type-2 diabetes and obesity</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2008

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

General Physiology and Biophysics

ISSN

0231-5882

e-ISSN

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Volume of the periodical

27

Issue of the periodical within the volume

2

Country of publishing house

SK - SLOVAKIA

Number of pages

9

Pages from-to

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UT code for WoS article

258040300003

EID of the result in the Scopus database

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