Compensation of illumination inhomogeneities in confocal laser scanning microscope (CLSM) images

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F09%3A00329250" target="_blank" >RIV/67985823:_____/09:00329250 - isvavai.cz</a>

Alternative codes found

RIV/68407700:21460/09:00156737

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Compensation of illumination inhomogeneities in confocal laser scanning microscope (CLSM) images

Original language description

Ideally, CLSM image regions corresponding to the same concentration of fluorescent material in the specimen should be mapped to the same grayscale levels. In practice, unfortunately, even ideally homogeneous specimens like artificial calibration grids exhibit darkening of image edges, lightening of the centre etc.; the effects are yet more pronounced in images of real biological specimens. Our solution to the problem is based on estimating a spatially variable gain which models the adverse effects of uneven illumination, and correcting images by inverting the estimated gain. The proposed approach exploits a morphological operation called the upper Lipschitz cover

Czech name

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Czech description

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Type

D - Article in proceedings

CEP classification

JC - Computer hardware and software

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2009

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Stereology and Image Analysis

ISBN

978-88-7488-310-3

ISSN

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e-ISSN

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Number of pages

6

Pages from-to

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Publisher name

Esculapio

Place of publication

Bologna

Event location

Milano

Event date

Jun 22, 2009

Type of event by nationality

EUR - Evropská akce

UT code for WoS article

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